CHANGES IN FRESH BEEF DURING COLD STORAGE. 
19 
Table 7. — Composition expressed in terms of percentage of moisture-free and 
fat-free material. 
Description of sample. 
Stor- 
age 
period. 
Incu- 
ba- 
tion 
nod. 
Mois- 
ture, 
fat-free 
basis. 
Ash. 
Total 
nitro- 
gen. 
Am- 
moni- 
acal 
nitro- 
gen. 
Phosphorus. 
Serial 
No. 
Total. 
Solu- 
ble. 
Insolu- 
ble. 
109 
Composite sample, left hind 
Hours. 
24 
24 
Days. 

7 
76.61 
76.66 
4.85 
5.01 
15.26 
15.69 
0. 0382 
.0815 
0.899 
.938 
0.718 
.836 
0.181 
110 
Sample No. 22 
.102 

7 
+0.05 
+0.16 
+0.43 
+.0433 
+.039 
+.118 
-.079 
Sample No. 17 
111 
24 

14 
14 
76.46 
-0.15 
4.96 
+0.11 
15.55 
+0.29 
.0996 
+. 0614 
.918 
+.019 
.855 
+.137 
.063 
-.118 
112 
24 

21 
21 
76.02 
-0.59 
5.09 
+0.24 
15.48 
+0.22 
.1084 
+.0702 
.947 
+.048 
.877 
+.159 
.070 
-.111 
Sample No. 12 
113 
24 

28 
28 
75.33 
-1.28 
4.58 
-0.27 
14.54 
-0.72 
.1464 
+. 1082 
.878 
-.021 
.811 
+.093 
.067 
—.114 
Sample No. 33 
120 
24 

42 
42 
74. 52 
-2.09 
4.73 
-0.12 
14.85 
-0.41 
.1478 
+. 1096 
.885 
-.014 
.835 
+.117 
.050 
Change 
—.131 
Sample No. 18 
121 
24 

64 
64 
75.28 
-1.33 
4.94 
+0.09 
14.33 
-0.93 
.2134 
+. 1752 
.872 
-.027 
.823 
+.105 
.049 
—.132 
Sample No. 32 
122 
24 

77 
77 
73.48 
-3.12 
4.71 
-0.14 
14.68 
-0.58 
.1868 
+. 1486 
.884 
-.015 
.850 
+.132 
.034 
-.147 
Sample No. 3 
124 
24 

93 
93 
73.49 
-3.13 
4.93 
+0.08 
14.30 
-0.96 
.2284 
+. 1902 
.875 
-.024 
.835 
+.117 
.040 
—.141 
Sample No. 2 
125 
24 

100 
100 
72.64 
-3.97 
4.76 
-0.09 
15.23 
-0.03 
. 2362 
+ . 1980 
.882 
-.017 
.867 
+.149 
.015 
Change 
—.166 
Table 8 shows the composition of the 0.9 per cent sodium clilorid 
extract of the meat expressed in terms of percentages of the original 
.material; but the changes in the composition of the extract are shown 
more clearly in Table 9, where the results are calculated in terms of 
percentages of the moisture-free and fat-free material. Table 9 
shows the composition of the 0.9 per cent sodium, clilorid extract of 
the samples expressed in terms of moisture- free and fat-free material. 
Total solids. — The data for total solids show some striking pecu- 
liarities. The samples incubated for 7, 14, and 21 days show de- 
creases amounting to 4.54, 2.89, and 3.75 per cent, respectively, while 
the samples incubated for periods ranging from 28 to 100 days 
show fairly regular increases varying from 3.62 to 8.77 per cent. 
The probable reasons for these irregular changes in total solids will 
be discussed in connection with soluble nitrogen, Table 10. 
Ash of extract. — There is a fairly marked increase in this constit- 
uent in the incubated samples as compared with the fresh material, 
but the extent of the increase bears no relation to the length of the in- 
cubation period. In the column headed " Total soluble phosphorus " 
increases may be noted in that constituent that go to confirm and 
explain the increases in ash of extract. The increase in ash of ex- 
tract is apparently due, in large part at least, to the change of insolu- 
ble phosphorus compounds (probably organic) to soluble forms. 
The cause and nature of these changes will be discussed in connec- 
tion with changes taking place in the phosphorus compounds. 
