CHANGES IN FEESH BEEF DURING COLD STORAGE. 15 
shaking the apparatus for 2 minutes each at the beginning, in the middle, 
and at the end of the reaction period. 
The results of this determination are reported in terms of percentages of 
elementary nitrogen. 
Acidity was determined by titrating 100 c. c. of the extract against tenth- 
normal sodium hydroxid, using phenolphthalein as an indicator. Results are 
calculated in terms of lactic acid. 
Total soluble phosphorus. — For this determination, 100 c. c. of the 0.9 per 
cent sodium chlorid extract was used. The method of determination was iden- 
tical with the method used for the estimation of total phosphorus. 
Soluble inorganic phosphorus was determined by the method of Chapin and 
Powick (1915), which consists essentially in the removal of the protein mate- 
rial by means of picric acid and the subsequent double precipitation of the 
inorganic phosphorus from an aliquot of the filtered picric acid solution, first, 
as magnesium ammonium phosphate and afterwards as ammonium phospho- 
molybdate according to the method of Lorenz (1901). "Modification B" of 
this method was used, no correction being made for the volume of the picric 
acid coagulum. For the sake of brevity the details of this method must be 
omitted. A complete description will be found in the original article by Lorenz. 
The results are reported in terms of percentages of elementary phosphorus. 
Soluble organic phosphorus. — The percentage of soluble organic phosphorus 
was obtained by subtracting the percentage of soluble inorganic phosphorus 
from that of total soluble phosphorus. 
COMPOSITION OF DIFFERENT MUSCLE BUNDLES FROM A HIND QUARTER OF 
A STEER. 
The original plan for the conduct of the autolysis experiment was 
to determine the changes taking place in individual bundles of 
muscles; but in practice it was found in the course of some unre- 
ported work that it would be impracticable to obtain a sufficient 
number of sterile samples by this procedure. This plan was there- 
fore abandoned and it was decided to take the samples for incubation 
at random from the muscular portions of the round of the hind 
quarter, and then to determine the composition of the fresh material 
by analyzing a composite sample taken from many parts of the round. 
It was recognized that there might be slight diiferences in the 
composition of the different muscle bundles, a fact that would need 
to be taken into consideration in interpreting the results of an autoly- 
sis experiment conducted according to our plan. For this reason 
the composition of five of the more important bundles of muscles 
from the round of a fat steer was determined. The quarter of beef 
had been held in cold storage at 32°-34° F. for 48 hours previous to 
dissecting out the muscle bundles, and samples prepared for analysis 
were held in cold storage for an additional period of 24 hours, 
making a total of 72 hours' storage before analytical work was 
started. 
Tables 1 to 5 inclusive show the composition of five muscle bundles 
from the hind quarter of the steer. Tables 1 and 3 show the com- 
