42 
BULLETIN 780, U. S. DEPARTMENT OE AGRICULTURE. 
AT REFRIGERATOR TEMPERATURE. 
In Table XXIV are summarized experiments the results of which 
indicate the approximate period during which Nosema apis remains 
virulent in the bodies of infected bees kept at refrigerator temper- 
ature. 
Table XXIV. — Resistance of Nosema apis in dead bees drying at refrigerator temperature. 
Date of inoculation. 
Period in 
refrig- 
erator. 
Results of inoculation. 
Mar. 4,1916. 
Mar. 20, 1916 
Mar. 20. 1916 
Apr. 22.1916. 
May 6, 1916.. 
Dec. 7,1915. 
Jan. 7, 1916.. 
May 24, 1916. 
June 3, 1916. 
Feb. 10, 1916 
Apr. 7, 1916. 
Mar 6, 1916. 
July 3, 1916. 
Nosema infection produced. 
Do. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
The results recorded in Table XXIV show that Nosema apis in 
the bodies of dead infected bees remained virulent at refrigerator 
temperature from two and a half to four months. 
ON THE SOIL. 
Dead Nosema-infected bees were placed on the soil in the open, 
but in a somewhat shaded spot. After different intervals of time 
colonies were inoculated, these dead, bees being used as the source 
for the infective material. Table XXV summarizes the experi- 
ments performed, the results of which indicate the approximate 
period during which Nosema apis remains virulent in the bodies of 
dead bees lying on the soil. 
Table XXV. — Resistance of Nosema apis in dead bees lying on the soil. 
Date of inoculation. 
Results of inoculation. 
July 16, 1915. 
Aug. 1, 1916. 
Aug. 28, 1915 
Aug. 12, 1916 
Aug. 26, 1916 
Aug. 28, 1915 
May 14, 1916. 
Oct. 4, 1915.. 
Oct. 21,1915. 
Oct. 21, 1915. 
Oct. 4, 1915.. 
Nov. 9, 1915. 
Nosema infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
