NOSEMA-DISEASE. 
45 
In the experiments honey was diluted with an equal quantity of 
water and medicated. 1 To the medicated solution Nosema apis was 
added. This suspension was fed to a colony, usually within a half 
hour from the time it was made. On each of four or five days imme- 
diately following the inoculation, the colony was fed honey medicated 
with the drug but free from Nosema apis. 
In Table XXVIII are summarized the experiments performed, 
together with the results obtained. 
Table XXVIII. — Effect of drugs on Nosema infection. 
Drug. 
Experiment 1. 
Experiment 2. 
Experiment 3. 
Experiment 4. 
Propor- 
tion. 
Results. 
Propor- 
tion. 
Results. 
Propor- 
tion. 
Results. 
Propor- 
tion. 
Results. 
Betanaphthol 
Salol 
2:1,000 
2:1,000 
2:1,000 
3:1,000 
3:1,000 
5:1,000 
10:1,000 
No infec- 
tion. 
...do 
...do 
...do 
...do 
Infection. 
...do 
1: 1,000 
1:1,000 
1:1,000 
2:1,000 
2:1,000 
4:1,000 
4:1,000 
No infec- 
tion. 
...do 
...do 
...do 
...do 
Infection. 
...do 
1:2,000 
1:2,000 
1:2,000 
1:1,000 
1:1,000 
2:1,000 
2:1,000 
No infec- 
tion. 
...do 
Infection. 
...do 
...do 
...do 
...do 
1:5,000 
1:5,000 
1:5,000 
Infection. 
Do. 
Salicylic acid 
Carbolic acid 
Formic acid 
Eucalyptus 
Do. 
The results recorded in Table XXVIII show that the parasite was 
destroyed by some of the drugs used but that it resisted others. 
Their relative efficiency as indicated from these preliminary results 
is shown by the arrangement in the table. Betanaphthol and salol 
seem to be the most effective of those tried, and eucalyptus and 
quinin the least efficient. 
Experiments were performed in which the inoculation with Nosema 
apis was not followed by feedings with medicated sirup. The 
results obtained show that under the conditions of the experiments 
the drugs affected the parasite as seen by the lower percentage of 
Nosema-infected bees in the colonies inoculated. In colonies re- 
ceiving subsequent feedings of medicated sirup a still lower percent- 
age of infected bees was found. 
While it is thus established that Nosema apis is somewhat sus- 
ceptible to the effects of some of the drugs, the experiments are 
altogether too few for definite conclusions as to the extent of their 
action. Statements regarding the effect of the drugs on Nosema- 
disease, therefore, should be accepted cautiously, for the present at 
least, unless they are supported by experimental or other good 
evidence. 
1 In obtaining the desired proportion of the drug, betanaphthol, salol, salicylic acid, and eucalyptus were 
dissolved in alcohol. In the- case of carbolic acid, formic acid, and the bisulphate of quinin aqueous 
solutions of the drugs were employed. 
