NOSEM A-DISE ASE . 4 1 
Table XXII. — Resistance of Nosema apis in dead bees hept at room temperature. 
Date of inoculation. 
Results of inoculation. 
Aug. 4, 1915. 
Aug. 10, 1915 
July 17, 1916. 
Aug. 26, 1916 
Aug. 17, 1916 
June 3, 1916. 
Aug. 23, 1915 
July 26, 1916 
Aug. 1,1916. 
June 27, 1916 
Aug. 20, 1914 
Aug. 30, 1914 
Nosema infection produced. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
From Table XXII it is learned that when dead infected bees were 
kept at room temperature the parasite remained virulent for three 
or four weeks, but did not produce the disease after one month. 
Since the temperature of the room was not constant, variations in 
results obtained at this temperature are to be expected. 
AT OUTDOOR TEMPERATURE. 
Dead Nosema-infected bees were placed in a hive body standing in the 
experimental apiary. At different intervals suspensions were made 
and colonies were inoculated. In Table XXIII are summarized a 
few experiments indicating by the results obtained the approxi- 
mate period Nosema apis remains virulent in the body of dead bees 
at outdoor temperature. 
Table XXIII. — Resistance of Nosema apis in dead bees drying at outdoor temperature. 
Date of inoculation. 
Period of 
drying. 
Results of inoculation. 
Oct. 19,1914. 
Aug. 23, 1915 
Nov. 2, 1914. 
June 7, 1916. 
June 27, 1916 
July 17,1916. 
Days. 
28 
35 
42 
36 
56 
76 
Nosema infection produced. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
From Table XXIII it is seen that Nosema apis in the bodies of 
dead infected bees kept dry at outdoor temperature remained viru- 
lent for from five to six weeks. These experiments extended over a 
period from June to November, as shown by the dates. It is to be 
expected that if they had been conducted throughout the year the 
results obtained would have shown a much wider range of variation. 
