34 
BULLETIN" 780, U. S. DEPARTMENT OE AGRICULTURE. 
From experiments recorded in Table XII it was shown that Nosema 
apis was destroyed by fermentation in 20 per cent honey solution at 
incubator temperature in three days. 
FERMENTATION AT ROOM TEMPERATURE. 
In Table XIII are summarized experiments in which colonies were 
inoculated with a suspension of Nosema apis in a 10 per cent sugar 
(saccharose) solution, which had been allowed to ferment, at room 
temperature. 
Table XIII. — Resistance of Nosema apis to fermentation in sugar solution at room 
temperature. 
Date of inoculation. 
Results of inoculation. 
Sept. 8, 1915. 
Sept. 9, 1915. 
June 4, 1915.. 
Sept. 10, 1915 
July 27, 1915. 
July 29, 1915. 
Sept. 13, 1915 
Sept. 15, 1915 
Sept. 16, 1915 
Sept. 1,1915. 
Jan. 9, 1915.. 
Sept. 15, 1914 
Sept. 29, 1914 
June 9, 1914.. 
June 10, 1914. 
May 13, 1915. 
Nosema infection produced. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
From Table XIII it will be seen that the parasite was destroyed 
by fermentation in a 10 per cent sugar solution at room temperature 
in from 7 to 1 1 days. The range of variation shown may be attributed 
largely to variation in the temperature. 
FERMENTATION AT OUTDOOR TEMPERATURE. 
In Table XIV are summarized experiments made for the purpose 
of obtaining approximate data relative to the resistance of Nosema 
apis in a 20 per cent honey solution at outdoor temperature. 
Table XIV. — Resistance of Nosema apis to fermentation in a honey solution at outdoor 
temperature. 
Date of inoculation. 
Period of 
fermen- 
tation. 
Results of inoculation. 
July 26, 1916. 
July 27, 1916. 
July 28, 1916. 
Sept. 8, 1915. 
July 29. 1916. 
Aug. 30, 1916 
July 29, 1916. 
Aug. 31, 1916 
Sept. 2, 1916. 
Sept. 6, 1916. 
Nosema infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
