32 BULLETIN 780, U. S. DEPARTMENT OF AGRICULTURE. 
Table IX. — Resistance of Nosema apis to drying at room temperature. 
Date of inoculation. 
Period of 
drying. 
Results of inoculation. 
July 26 , 1916 
Days. 
18 
21 
35 
42 
43 
50 
56 
60 
61 
95 
Nosema infection produced. 
Do 
Sept. 11, 1914 
Aug. 11, 1916 
Do 
Oct. 2, 1914 
Do 
Sept. 1, 1915 
Do 
Aug. 26, 1916 
Do 
Oct. 16, 1914 
Do 
May 24, 1916 
No infection produced. 
Do 
Sept. 1,1915 
June 27, 1916 
Do 
From results recorded in Table IX it will be observed that Nosema 
apis drying at room temperature remained virulent for from 56 to 
60 days, that is, about 2 months. 
NOSEMA APIS DRYING AT OUTDOOR TEMPERATURE. 
Table X summarizes experiments in which Nosema apis was allowed 
to dry at outdoor temperature. 
Table X. — Resistance of Nosema apis to drying at outdoor temperature. 
Date of inoculation. 
Period of drying. 
Months. 
Days. 

21 

42 

46 

56 

60 

60 

75 

80 

85 

100 
9 

10 
10 
11 

Results of inoculation. 
Sept. 11, 1914 
Oct. 2, 1914.. 
Aug. 17, 1914 
Oct. 16, 1914. 
June 7, 1916. 
Sept. 27, 1913 
July 9, 1915.. 
June 27, 1916 
Aug. 17, 1915 
July 17, 1916. 
May 25, 1915. 
July 17, 1914. 
July 1,1914.. 
Nosema infection produced. 
Do. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
The results recorded in Table X show that Nosema apis ceased to 
be virulent after 2 months of drying at outdoor temperature. 
NOSEMA APIS DRYING AT REFRIGERATOR TEMPERATURE. 
Table XI summarizes experiments in which Nosema apis was 
allowed to remain dry in the refrigerator. 
Table XI. — Resistance of Nosema apis to drying at refrigerator temperature. 1 
Date of inoculation. 
Results of inoculation. 
Dec. 2, 1915. 
Jan. 3, 1916 . 
Mar. 3, 1916. 
Apr. 2, 1916. 
Apr. 22, 1916 
May 3, 1916. 
July 3, 1916. 
Nosema infection produced. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
1 A few times during the experiments in which the refrigerator temperature was used, the ice became 
exhausted, allowing the temperature to approach and possibly to reach that of the room. This higher 
temperature, when present, however, at no time prevailed for more than a day. 
