NOSEMA-DISEASE. 
31 
Table VII shows that Nosema apis in a honey suspension was 
destroyed by heating for 10 minutes at a temperature between 136° F. 
(58° C.) and 140° F. (60° C), the death point being about 138° F. 
(59° C). 
RESISTANCE OF NOSEMA APIS TO DRYING. 
In experiments relative to the effect of drying on Nosema apis, 
stomachs from Nosema-infected bees were crushed, and the crushed 
tissues were smeared on slides to the extent of a thin layer. The 
slides were placed in incubator, room, outdoor, and refrigerator tem- 
peratures, respectively. At different intervals after the preparation 
of the smears an aqueous suspension was made, germs from two slides 
representing the material from 5 to 20 bees being used. This was 
added to sirup and fed to a healthy colony. Whether or not the 
parasite had been destroyed was determined by the presence or 
absence of Nosema-infection in the colony following the inoculation 
with the sirup. 
NOSEMA APIS DRYING AT INCUBATOR TEMPERATURE. 
In Table VIII are summarized the experiments, together with the 
results obtained, in which the Nosema material was allowed to dry at 
incubator temperature. 
Table VIII. — Resistance of Nosema apis to drying at incubator temperature. 
Date of inoculation. 
July 30, 1915. 
July 14, 1916. 
Oct. 5, 1914.. 
July 21, 1915. 
July 29, 1916. 
Sept. 11, 1914 
Nov. 2, 1914.. 
Sept. 29, 1914 
Oct. 16, 1914. 
July 9, 1915 . . 
May 24, 1915. 
July 9, 1915.. 
Period of drying. 
Months. 









2 
7 
9 
Days. 
10 
13 
14 
18 
15 
21 
30 
40 
56 
15 
27 
19 
Results of inoculation. 
Nosema infection produced. 
Do. 
Do. 
Do. 
No infection produced. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
From Table VIII it will be seen that Nosema apis drying at incu- 
bator temperature was destroyed in from 15 to 21 days, that is, 
during the third week. 
NOSEMA APIS DRYING AT ROOM TEMPERATURE. 
In Table IX are summarized experiments in which the drying of 
Nosema apis took place at room temperature. 
