NOSEMA-DISEASE. 13 
As is pointed out under the heading "Climate," Nosema-disease 
occurs in a wide range of localities. The food and water obtained in 
these localities naturally differ as to quality and quantity. Infection 
is found in colonies having an abundance of stores and in others 
having a scarcity. The disease is produced readily by experimental 
inoculations in colonies with much and in colonies with little stores. 
From these observations the conclusion seems to be justified that 
the role played by food in the causation of Nosema-disease is slight, if 
indeed it contributes at all appreciably to it. 
A THREE-YEAR STUDY OF NOSEMA INFECTION IN AN APIARY. 
The presence of Nosema infection among bees in the apiary of the 
Bureau of Entomology was discovered in May, 1910 (White, 1914) 
In April, 1912, a more or less systematic study was begun on the prev- 
alence and persistence of the infection in the apiary and was con- 
tinued until June, 1915, As the apiary was being used for other 
purposes than these studies, it was not possible to follow all of the 
colonies throughout this entire period. In Table II are summarized 
observations made during the first year of the study. 
