VITAMIN B IN EDIBLE TISSUES OF OX, SHEEP, AND HOG. 35 
40 minutes at 210°-220° C. The cooked heart was then ground and 
dried. Pen 64 was fed a ration containing 15 per cent of the cooked 
ox heart.. Two pigeons developed polyneuritis, one on the twenty- 
second day, the other on the thirty-seventh day, and the other two 
birds were in good condition at the close of the test on the fifty-ninth 
day. By comparing the result of this test with that secured from 
feeding the-ration that contained 15 per cent of uncooked ox heart, 
pen 45, Table 16, it is evident that the method employed in cooking 
the ox heart materially lowered its antineuritic value. 
The ox kidney was cooked in the following manner: The fresh kid- 
neys were split open, trimmed as free as practicable from fat and con- 
nective tissue, cut into small cubes, and then washed thoroughly in 
cold water. The kidney was then placed in a kettle, covered with 
water, and heated to boiling. The water was poured off, fresh water 
was added, and boiling was continued for an hour when the kidney 
was sufficiently tender to eat. The water was poured off and the 
cooked kidney was ground and dried is the usual manner. Pens 65 
and 66 were fed rations which contained 15 and 25 per cent, respec- 
tively, of the cooked ox kidney. The ration containing 15 per cent 
of the cooked ox kidney had a rather low antineuritic value. Three 
pigeons developed polyneuritis on the twentieth, twenty-fourth, and 
fiftieth days, respectively, and one bird was in very poor condition at 
the close of the test on the fifty-sixth day. The ration that contained 
25 per cent of the cooked kidney had a fair antineuritic value. One 
pigeon developed polyneuritis on the fifty-fourth day; the three others 
were in fair condition at the close of the test on the fifty-sixth day. 
The pen of birds suffered an average loss in weight of 13 per cent, 
which is an indication of a deficiency of the antineuritic vitamin in 
the ration. 
TESTS WITH OX BRAINS AND LAMB BRAINS. 
The results of the feeding test with ox and lamb brains are reported 
in Table 22. The ration containing 15 per cent of ox brains had a 
very low antineuritic value, three of the birds having developed 
polyneuritis by the nineteenth day and the fourth bird was in poor 
condition at the close of the test period of 55 days. The average 
survival period was 26 days and the loss in weight 9.7 per cent. 
The ration which contained 25 per cent of ox brains had a fair anti- 
neuritic value, the average survival period being 41 days and the loss 
in weight 6.7 per cent. ‘Two birds developed polyneuritis on the 
twenty-fifth and thirtieth days, respectively; the other two were in 
good condition at the close of the test. 
Lamb brains had practically the same antineuritic value as ox 
brains. One bird in pen 70 died on the twenty-second day of the 
test on account of an injury. The changes in the weights of the 
pecans getting the ox-and-lamb brains ration are shown in Figures 
30 and 36. 
