VITAMIN B IN EDIBLE TISSUES OF OX, SHEEP, AND HOG. 33 
Pen 60 was fed a ration containing 30 per cent of ox liver that had 
been heated two hours in an autoclave at 124° C. The previously 
dried liver was spread out in a thin layer in pans and heated in an 
autoclave under the conditions stated, and then dried in the usual 
way. The result of the feeding test with this lot of liver indicated 
that the antineuritic properties of the tissue had been practically 
destroyed. . The four pigeons on this ration developed polyneuritis 
on the seventeenth, twenty-third, twenty-fourth, and twenty-fifth 
days, respectively. The average survival period was 22 days, and 
the average loss in weight was 18 per cent. 
PEN SI FEN COO PEN 6/ 
JO PER CENT LIVER JOPER CENT LIVER JO PER CENT 
COOKED 2 HOWRS 777 COOKED 2 HOURS OX LIVER COOAELD £2 
4OO DEGREES C/V AIT 124 DEGREES C HOURS AIT 430 LECKLES 
STLAW7 IN AIM AUTOCLAVE CIN AWN AUTOCLAVE 
Scape ote alae EUs nN I 
400 | J a fe [cal Eo] Talo 
Caan see a Zee aes 
Ovo Sissel ech a 
wenrc eal QiB@eccuneaNee 
Ree AN u peeale ot eNe | Nise eleey 1 
vee HENS Rae CaaS FS ea wee 
poe ee ee NP el Nhe 
ese eal | aA ES Fhe Sale aha 
B90 WO 
220 
=e Fede GCS OGESRROReRne, NEN 
Fe ls ap ES a a 
LACH) HORIZONTAL SKFYICE REPRESENTS /O DPFIYS - 
Fig. 32.—Cooked ox liver; changes in weights of pigeons fed. 
SLI OS 
PEN O62 <b FER CENT LIVER 
ane OLAV BSAIKLOD 2 HOURS 
FRIED LIVER 747 186 DEGREES C 
+ 
} 
| 
| | jal i 1 | 
<4 0 EH HORIZONTAL SFAICE REPRESENTS 10 L795. 
Fic. 33.—Fried and baked ox liver; changes in weights of pigeons fed. 
Pen 61 was fed a ration which contained 30 per cent of ox liver 
that had been heated 2 hours at 130° C. in an autoclave. The anti- 
neuritic properties of this lot of liver were largely destroyed by the 
method of cooking used. 
Pen 62 was fed a ration containing 25 per cent of ox liver that had 
been fried in the manner customary in its preparation for the table. 
The fried liver was ground and dried. This pen of pigeons was fed 
for a period of 70 days and the average survival period was 59 days. 
Three pigeons developed polyneuritis on the forty-third, forty-eighth, 
and sixty-fifth days, respectively, while two birds finished the test in 
fair condition. By comparing the results obtained from this pen of 
