VITAMIN B IN EDIBLE TISSUES OF OX, SHEEP, AND HOG. 23 
PREVIOUS INVESTIGATIONS WITH EDIBLE VISCERA. 
Cooper (6) (15), (1912) (1914) studied the antineuritic properties 
of a number of animal tissues in feeding tests with pigeons. Ox liver 
had the highest value, the daily addition of 0.9 grams of the dry 
tissue to the ration of pigeons being sufficient to protect them against 
polyneuritis for 50 days. The following quantities of each of the 
other tissues tested had like values: Ox heart, 1.7 grams; ox cere- 
brum, 1.2 grams; ox cerebellum, 2.4 grams; and sheep cerebrum, 1.6 
to 3 grams. 
McCollum and Davis (16) (1915) report that the addition of dried 
pig heart or kidney to the “fat-free diet”’ of rats that were declining 
im weight greatly stimulated growth, the kidney having greater value 
than the heart. 
Eddy (17) (1916) found that the water-soluble portion of an alco- 
holic extract of sheep pancreas was capable of inducing marked 
erowth in rats that had previously been fed a vitamin-free diet. 
Osborne and Mendel (18) (1918) studied the value of dried pig 
heart, liver, kidney, and brain in the diets of young rats as a source 
of vitamins A and B, as well as of protem. They found that 19 
per cent of pig heart in the ration as the sole source of protein and 
vitamins A and B induced normal growth in rats. Similar results 
were obtained with a ration containing 22 per cent of pig kidney. 
Rats made normal growth on a ration that contained 32.5 per cent 
of pig brain as a source of vitamin B, vitamin A being supplied in 
the form of butterfat. Ten per cent of pig brain did not supply 
sufficient vitamin B for growth. Seven per cent of pig liver, in an 
otherwise adequate diet, did not furnish sufficient vitamin B for 
erowth; but when 10 per cent of liver was added, satisfactory growth 
took place. | 
McCollum, Simmonds, and Parsons (10) (1921) found that 25 per 
cent of dried ox liver or of ox kidney in a ration furnished an ample 
supply of vitamins A and B for growth and reproduction in white 
rats. Rats receiving 20 per cent of ox kidney in the ration also 
grew and reproduced normally, but those getting 20 per cent of ox 
liver did not do so well. One lot of rats that was fed solely on dried 
ox blood declined rapidly in weight and at the end of two weeks the 
ration was changed to contain 50 per cent of dried ox muscle. The 
rats then grew at about half the normal rate. 
EXPERIMENTAL WORK. 
The method of procedure followed in these experiments was prac- 
tically the same as that employed in the tests with muscle tissue 
described in Part I. The various tissues studied were obtained in 
fresh conditions from local meat-packing establishments and were 
dried in the manner previously described. The dry tissue was used in 
all tests and the polished rice was first ground and then heated four 
hours at 120° C. in an autoclave before being used in the rations. 
TESTS WITH OX LIVER. 
The results of the feeding tests with ox liver are presented in Table 
13. Three pens of pigeons were fed rations which contained 5, 15, 
and 30 per cent, respectively, of ox liver. By comparing the survival 
