VITAMIN B IN EDIBLE TISSUES OF OX, SHEEP, AND HOG. 7 
ofjhigh antineuritic value is heated in an autoclave for two hours at 
130° C. its protective properties are practically destroyed. For these 
reasons all the polished rice which was used in subsequent experi- 
ments as a base in the rations with muscle, was heated two hours at 
130° C. in an autoclave. The change in weight of the pigeons in 
pens 1, 2, and 3 is shown graphically in Figure 4, at the end of this 
paper. The marked and rapid decrease in the weights of the birds 
is.at once apparent. 
Vi aay Np VED NEST 
KICE HEATER RICE HEATED 
2 HOURS IN  2HOYRS 1/V 
EN / AUTOCLAVE AT AUTOCLIYUE AT 
UNTREATED RICE VGae VCE 
420; ES = Fae Serna te ae 
SO) ae ae 
360 1+ + 
\ 340) aa jean ee 
S520 4 he N 
\ Sig) ee See 
§ 260/44 
260 : aan Ho 
AO res oie aa al j —- +4 + | = 
220 + iP ertealie NG aia bs Nets fra 
200-1 ; aie pares! ~\ ) 
/3O oP SEH Se | ee {er SS fo Cc Sie | [emer oe 
EAICH HORIZONTAL. SPACE REPRESENTS /@ DAYS 
Fic. 4.—Variations in weight of pigeons resulting from feeding polished rice. The 
numbers opposite beginnings of lines refer to Pigeon Nos. of tables. 
The graphs presented in Figures 4 to 45, inclusive, are based upon 
the weights of the pigeons recorded at approximately weekly intervals 
during the experiments, except that the weight of any bird suffering 
from a congestion of food in its crop, an early symptom of poly- 
neuritis, was not recorded. Since most pigeons that develop poly- 
neuritis suffer from this condition of the crop, it was found to be 
inadvisable to weigh each bird at the time it developed polyneuritis. 
For this reason, then, the graph of a pigeon usually indicates a shorter 
period than the survival period for the same bird recorded in the 
table. Occasionally a graph may indicate a slightly longer period 
than the survival period shown in the table. In such cases the crop 
of the bird was normal and the last weighing was made a few days 
after the development of the disease. The percentage change in 
weight of each pigeon, as indicated in the tables, is based upon the 
initial weight and upon the last normal weight as shown in the graph 
for the same bird. The graphs are presented distinctly for the pur- 
pose of showing at a glance the rate and extent of the change in 
weights of the pigeons during the experiments, and not for the pur- 
pose of indicating the survival periods of the birds. 
TESTS WITH OX MUSCLE. 
The results of the feeding tests with ox muscle are shown in Tables 
2, 3, and 4, and the changes in the weights of the birds are shown 
eraphically in Figures 5, 6, 7, 8, 9, and 10. 
Pens 4 and 5 (Table 2) were fed rations containing 25 and 15 per 
cent, respectively, of muscle derived from the round of the carcass 
of a single fat steer. The slight difference in the average survival 
periods of the two pens of birds is not material. There was prac- 
