MAINE SARDINE INDUSTRY. 45 
would contain 2,509 grams, or 5.5 pounds, of salt. Subtracting this 
amount of salt from the total actual loss during the 20-hour period 
gives 12.9 pounds, or 18.4 per cent, of water and protein material 
removed from the original quantity of fish. The same calculation 
applied to the results obtained for the 12-hour period indicates that 
9.3 pounds, or 13.3 per cent, of water and protein material was 
extracted from the fish during this time. 
The second experiment of this series was run on fish which had 
been but 3 hours out of the water, being, therefore, somewhat fresher 
than those used in the first experiment. They were of uniform size, 
about 7 inches long, and were much fatter than those of the previous 
lot. One hundred and twenty-four pounds of fish were treated in the 
same manner as the others (p. 40), using 23 pounds of salt. The results 
of analysis are recorded under “ Experiment 2” in Table 17. 
During the 12 hours they remained in salt these fish lost the same 
percentage of water as those in Experiment 1. A comparison of 
results for the two experiments shows that the fatter fish do not 
absorb salt quite as rapidly as the thinner ones. A more gradual 
absorption of salt up to, and including, the 8-hour period and a more 
marked increase during the 10- and 12-hour periods occurred in the 
fish used for Experiment 2. The percentage amount absorbed dur- 
ing the 12 hours, however, was practically the same for both lots of 
fish. The greatest quantity of ammonia and amines was found in 
the fish at the end of the 4-hour period. 
The largest amount of brme was formed during the 2- to 4-hour 
interval. A decrease in the rate of formation of brine occurred when 
the brine was allowed to accumulate. The specific gravity of the brine 
was highest at the end of the 2-hour period. The greatest quantity 
of nitrogenous material extracted, represented by total nitrogen, 
was found at the end of the 4-hour period. The quantity of amino 
acid nitrogen was also correspondingly high for this period. In Ex- 
periment 1 the highest amount of protein material was extracted 
during the same interval of time, but the largest volume of brine 
was obtained at the end of the 6-hour period. The greatest quantity 
of ammonia and amines, as nitrogen, in the brine was found during 
the 10-hour period. 
With the exception of the specific gravity of the brine and the 
results for ammonia and amines, the quantity of the materials de- 
termined declined gradually from the fourth period, when the maxi- 
mum. was reached, to the end of the 12-hour period. For the entire 
12-hour period the total quantity of nitrogen in the brine was extracted 
at the rate of 151.1 mg. per pound of fish. None of the extractive 
materials was determined in the brine collected at the end of the 
20-hour period. 
