34 
BULLETIN 227, U. S. DEPARTMENT OF AGRICULTURE. 
Table IV. — Toxicity of various preservatives to certain wood-destroying and other fungi- 
Continued. 
Toxic substance. 
Organism. 
Toxic point. 
Culture 
medium. 
Duration of 
test. 
Investigator. 
B .—ORGANIC COMPOUNDS— 
continued. 
(b) Tars and creosotes — 
Continued. 
Coal-tar creosote— Contd. 
With 10 per cent fil- 
tered tar added. 
With 20 per cent fil- 
tered tar added. 
Coal tar (undistilled, sp. 
gr. 1.194 at 15.5° C.). 
Coal-tar creosote: 
Fraction I (sp. gr. 
0.934 at 60° C. 78.3 
per cent distills be- 
low 215° C). 
Do 
Penicillium 
do 
Molds 
Per cent. 
0.30 
0.60 
Between 1.5 
and 2. 
0.30 
0.225 
0.225 
0.15 
0.325 
0.125 
3.3 
0.125........ 
33 
7.8 
0.35 
0.25 
Between 4 
and 4.5. 
Above 10 
Agar.... 
...do 
...do 
...do 
...do 
...do 
...do:.... 
...do 
...do 
...do 
...do 
...do 
...do 
...do 
...do 
...do..... 
...do 
4 weeks 
do 
do 
4 to 6 weeks. 
do 
5 to 9 weeks. 
4 to 5 weeks. 
4 to 8 weeks. 
4 to 7 weeks. 
4 to 6 weeks. 
5 to 8 weeks. 
4 to 6 weeks. 
5 to 8 weeks. 
4 weeks 
do 
do 
do 
do 
do 
do 
J. M. Weiss. 
Do. 
Do- 
Fomes annosus 
Humphrey and 
Fleming. 
Do. 
Fraction II (sp. gr. 
1.003 at 60° C, 9 per 
cent distills below 
205° C, 95.9 per 
cent below 287° C). 
Do 
F. annosus 
F. pinicola 
F. annosus 
F. pinicola 
Do. 
Do. 
Fraction III (sp. gr. 
1.045 at 60° C, 73.7 
per cent distills be- 
low 295° C). 
Do 
Do. 
Do. 
Fraction IV (sp. gr. 
1.088 at 60° C 54.3 
per cent distills be- 
tween 285° and 320° 
%o 
F. annosus 
F. pinicola 
Do. 
Do. 
Fraction V (10 per 
cent distills above 
320° C, 36.6 per 
cent hard residue). 
Do 
Do. 
F. pinicola. 
Penicillium 
do 
do 
do. 
Do. 
Fraction below 235° C. 
Fraction between 
235°-272° C. 
Fraction above 272° C. 
Anthracene, pure 
J. M. Weiss. 
Do. 
Do. 
Do. 
Naphthalene, pure 
"Quinolin, pure 
do 
do 
do 
Between 9 
and 10. 
0.10 
Above 10. . . . 
0.40 
...do 
...do 
...do 
...do 
Do. 
Do. 
Do. 
Water-gas tar distillate: 
Fraction between 
Polystictus versicolor 
Penicillium 
do 
Dean and 
170°-340° C. 
Fraction between 
170°-315° C. (sp. gr. 
1.024 at 15.5° C). 
Fraction between 
2 
Above 10.... 
Above 40 
Above 40 
About 0.65 . . 
0.65 
0.20 
1.25 
0.75 
Above 10. . . . 
...do 
...do 
...do 
-..do 
...do 
...do 
...do 
...do 
...do 
...do 
4 weeks 
do 
4 to 8 weeks. 
4 to 5 weeks. 
5 to 8 weeks. 
4 to 7 weeks. 
4 to 6 weeks. 
do 
4 to 5 weeks. 
4 weeks 
Downs. 
J. M. Weiss. 
Do. 
210°-315° C. (sp. gr. 
1.053 at 15.5° C). 
United Gas Improve- 
ment Co. oil (sp. gr. 
1.058 at 60° C). 
Do 
Fomes annosus 
Humphrey and 
Fleming. 
Do. 
Sp. gr. 0.995 at 60° C. 
Wood creosote (softwood, 
sp. gr. 1.052 at 60° C— 
about 8 per cent H2O). 
Do 
Do. 
....do 
F. pinicola 
F. annosus 
F. pinicola 
Penicillium 
Do. 
Do. 
Wood tar (hardwood, sp. 
gr. 1.195 at 60° C). 
Do 
Do. 
Do. 
Semiasphaltic petro- 
J. M. Weiss. 
leum (sp. gr. 0.973 at 
15.5° C: distills above 
315° C). 
In conclusion, the writers wish to emphasize that any scale of 
toxicities derived from Petri-dish tests on the usual nutrient agar or 
