THE TOXICITY TO FUNGI OF VARIOUS OILS AND SALTS. 
23 
Coal-tar creosote, Fraction IV. 
(PI. II, figs. 14 to 19.) 
[Laboratory sample No. 1108. Cooperator, Semet-Solvay Co., Ensley, Ala. Liquid at room temperature; 
specific gravity, 1.088 at 60° C; flash point, 130° C; burning point, 136° C; 0.9 per cent distills below 
245° C; 54.3 per cent distills below 320° C] 
Tests. 
Times 
killing 
point 
verified. 
Concentration of the preservative (per cent). 
Fiuigus. 
Num- 
ber. 
Dura- 
tion. 
O 
d 
o 
o" 
o 
d 
d 
in 
d 
d- 
OS 
d 
— 
<N 
CO 
CO 
CO 
o 
75 
23 
Weeks. 
5 to 12 
5 to 8 
1 
1 
2 
2 
1 
1 
1 

4 
3 
2 
2 
1 

4 
1 The growth of Fomes annosus on concentrations above 2 per cent is very slight, usually not exceeding 
1 mm. in six weeks, and the actual toxic point is difficult to locate. It lies between 2.5 and 3.5 per cent. 
Fomes annosus: In 12 days, the radial growth of the check reached 25 mrn.; very 
slight growth on 0.2 to 0.5 per cent. In 18 days, 0.8 to 3 per cent reached 1 to 4 mm. 
In four weeks, 1.25 to 3 per cent reached 1 to 5 mm. The growth on the toxic media 
was usually darker than on the check. 
Fomes pinicola: In two weeks, the radial growth of the check reached 40 mm.; 
no growth on 0.025 per cent. In four weeks, 0.025 per cent showed 2 mm. ; no growth 
on higher concentrations. In eight weeks, 0.025 per cent reached 25 mm.; 0.05 per 
•cent, 15 mm.; 0.075 per cent, 10 mm.; 0.1 per cent, 2 mm.; no growth above this point. 
The growth on the" toxic cultures was dull, compact, and crinkled. 
Coal-tar creosote, Fraction V. 
(PL II, figs. 21 to 24.) 
[Laboratory sample No. 1109. Cooperator, Semet-Solvay Co., Ensley, Ala. Heavy, tarry liquid; specific 
gravity, 1.150 at 60° C; flash point, 172° C; burning point, 178° C; 10.1 per cent distills below 320° C; 
63.3 per cent distills below 380° C] 
Tests. 
Times 
killing 
point 
verified. 
Concentration of the preservative (per cent). 
Fungus. 
Num- 
ber. 
Dura- 
tion. 
0.1 
0.5 
1 
7 
7.8 
8 
30 
33 1 
Check. 
Fomes annosus 42 
F. pinicola 30 
Weeks. 
4 to 9 
4 to 8 


4 
3 
2 
2 
2 
1 
"""6" 
1 
1 

4 
4 
1 Those values are based on gum-arabic emulsions. 
Fomes annosus: In 10 to 14 days, the radial growth of the check reached about 
40 mm. ; 5.5 to 30 per cent showed 1 to 4 mm. In four weeks, 5.5 to 7.5 per cent reached 
8 to 15 mm. ; 15 and 25 per cent, 2 mm. ; no growth on 20 per cent gum-arabic emulsion. 
The growth on the toxic media was dense and creamy on the lower concentrations; 
submerged at first on the higher concentrations. 
Fomes pinicola: In two weeks, the radial growth of the check reached 40 mm.; 
in three weeks, 1 to 2 per cent showed initial growth; in four weeks, 3 to 6.5 per cent 
showed first growth; after eight weeks, 4.5 to 5 per cent reached 10 to 20 mm. The 
growth on the lower concentrations of the toxic media was white and fluffy;. on the 
higher concentrations it was tawny and radiate, with a crinkled edge. 
