22 BULLETIN 227, U. S. DEPARTMENT OF AGRICULTURE. 
Coal-tar creosote, Fraction II. 
(PL II, figs. 12 and 13.) 
[Laboratory sample No. 1106. Cooperator, Semet-Solvay Co.. Ensley, Ala. 
solid at room temperature: specific gravity. 1.003 at 60° C; flash point. 76 
9 per cent distills below 205° C. ; 95.9 per cent distills below 287° C] 
Naphthalene odor; nearly 
C: burning point. 85° C.r 
Tests. 
Times 
killing 
Concentration of the preservative (per cent). 
Fungus. 
Num- 
ber. 
Dura- 
tion. 
point j 
SSl oa °- 125 
0.15 0.175 0.2 
0.225 Check. 
20 
IS 
Weeks. 
5 to 9 
4 to 5 
1 
2 
, , 
4 4 
1 1 
4 
F. pinicola 
I 4 
1 
Fomes annosus: In two weeks the radial growth of the check reached 20 mm.; no 
growth on 0.15 per cent and above.. In four weeks, no growth on 0.225 per cent. In 
six weeks, 0.15 per cent reached 12 mm.; 0.2 per cent, 6 mm.; no growth on 0.225 
per cent. The growth on the toxic media was compact and creamy in color. 
Fomes pinicola: In two weeks the radial growth of the check reached 40 mm.; no 
growth on 0.125 per cent. In four weeks 0.1 and 0.125 per cent reached 40 mm. ; no 
growth above this point. The growth on the toxic media was a dingy white and less 
fluffy than that on the check. 
Coal-tar creosote, Fraction III. 
(PL III. fig. 30.) 
[Laboratory sample No. 1107. Cooperator, Semet-Solvay Co., Ensley, Ala. Liquid at room temperature,- 
specific gravity, 1.045 at 60° C; flash point, 103° C. ; burning point. 110° C; 0.9 per cent distills 1 elow 
215° C. ; 4.7 per cent distills below 2 75 ° C. ; fraction froml70° to 245° C. more or less solid with naphthalene.] 
Tests. 
Times 
killing 
i point 
verified. 
Concentration of the preservative (per cent). 
Fungus. 
Num.- Dura- 
ber. tion. 
1 
d , d 
d 
e 
«-J I "H <N 1 <N CO 
d d d 1 d i d 
CSJ 
d 
o 
6 
Weeks. 
60 4 to 12 
14 4 to 7 
2 
1 
.. Ill 
i 

4 
F. pinicola 
3 2 
o ..:J 
4 
i 
1 The radial growth of Fomes annosus on concentrations above 0.2 per cent is very slight, usually not 
exceeding 1 mm. , and the actual toxic point is difficult to locate. It lies between 0.25 and 0.35 per cent. 
Fomes annosus: In 19 days the radial growth of the check reached 40 mm. ; 0.15 and 
0.175 per cent, 1 or 2 mm. In five to six weeks 0.3 per cent showed 1 mm. and lower 
concentrations 1 to 5 mm. The growth on the toxic media was creamy and slightly 
lighter than that on the check. 
Fomes pinicola: In two weeks the radial growth of the check reached 40 mm. In 
about one month 0.05 per cent showed 3 mm.; 0.075 per cent. 2 mm. In about seven 
weeks 0.1 per cent showed initial growth: lower concentrations from 16 to 23 mm. 
The growth on the toxic media was a fluffy white on the lower concentrations: denser 
on 0.1 per cent. 
