THE TOXICITY TO FUNGI OF VARIOUS OILS AND SALTS. 
21 
Coal-tar creosote, grade C. 
(PI. I, figs. 5 to 9.) 
[Laboratory sample No. 1074. Purchased from the Creosote Supply Co., Chalmette, La. Liquid at room 
temperature, 8.4 per cent water; specific gravity, 1.0483 at 60° C; flash point, 93° C; burning point, 100° 
C; 11 per cent distills below 205° C. ; 54.1 per cent distills below 275° C. ; 74.1 per cent distills below 320° C] 
Fungus. 
Tests. 
It 
Concentration of the preservative (per cent). 
Num- 
ber. 
Dura- 
tion. 
o 
d 
C 
o 
d 
d 
d 
CO 
d 
d 
d 
d 
d 
M 
o 
O 
44 
21 
Weeks. 
4 to 8 
4 to 6 
1 
1 
2 
1 
1 

1 
1 
1 
1 
1 

4 
F. pinicola 
3 
2 
1 
4 
1 These values are based on gum-arabic emulsions. Later work, using the pure preservative, indicates 
that the toxic point may fall somewhat lower. 
Fomes annosus: In 17 days the radial growth of the check reached about 40 mm.; 
0.2 and 0.275 per cent showed initial growth only. In three to four weeks, 0.2 per 
cent showed 10 mm.; 0.25 to 0.5 per cent, 2 to 3 mm. In six weeks, 0.525 per cent 
showed only initial growth. In seven weeks, 0.5 and 0.525 per cent reached 2 to 9 
mm. The growth on the toxic media was about the same in appearance as on the 
check. 
Fomes pinicola: In two weeks the radial growth of the check reached about 40 mm. ; 
0.075 per cent, 3 mm.; no growth above this point. In four weeks, 0.075 per cent 
showed 25 mm. ; 0.1 per cent, 13 mm.; 0.15 per cent, 6 mm. After five weeks, 0.175 
and 0.2 per cent reached 3 mm.; above this point all growth was inhibited. The 
growth on the toxic media was dull white, with a crinkled edge. 
Coal-tar creosote, Fraction I. 
(PL II, figs. 10 and 11.) 
[Laboratory sample No. 1094. Cooperator. Semet-Solvay Co.. Enslev, Ala. Light liquid at room tem- 
perature; specific gravity, 0.934 at 60° C; flash point, 62° C; burning point. 69° C; 78.3 per cent dis- 
tills below 215° C] 
Fungus. 
Tests. 
Times 
killing 
point 
veri- 
fied. 
Concentration of the preservative (per 
cent). ' 
Num- 
ber. 
Dura- 
tion. 
0.2 
0.225 
0.25 
0.275 
0.3 
Check. 
Fomes annosus : 
33 
13 
Weeks. 
4 to 6 
4 to 6 
3 
1 
4 
4 
4 

2 
1 

4 
F. pinicola 
4 
Fomes annosus: In 10 to 14 days the radial growth of the check reached 20 mm.; 
0.2 to 0.25 per cent, from 1 to 4 mm. In four weeks, 0.2 to 0.225 per cent reached 30 
mm. ; 0.25 to 0.275 per cent, from 7 to 10 mm. ; no growth at 0.3 per cent. The toxic 
cultures produced a thin, stringy growth. 
Fomes pinicola: In two weeks the radial growth of the check reached 40 mm.; 
0.2 per cent, 1 mm. In four weeks, 0.2 per cent reached 40 mm.; no growth above 
this :»oint. The toxic cultures produced a fluffy white growth, similar in appearance 
to that on the check. 
