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22. BULEETEN 1012, U. S. 1 DEPARTMENT OF AGRICULTURE. 
times given in the table are computed from the time when. the 
feedings were finished. | : 
TasLe 8.—Time elapsed from feeding to development of symptoms. 
Animal. Time. ~ Animal. Time. 
Sheep Nos.: SEE Nos.—Contd. | 
AD eae ae eee ae l. Immediately... 9a. 2 nS 2 ae SO aes se ee Immediately. 
VG eee eee | 5 minutes. oe ia oe besa 6 hours. 
7. V Neier ae ee 12 minutes. HBB Ste eee le 3 hours. 
ZU VC n sl Foae Stee ene 28 minutes. : “de j99 Ree -....| 8 minutes. 
AA De Maras es eincers" Immediately. 6043.2 epee Se 2 hours, 24 minutes. 
AAG Be eA oc eH 14 hours, 55 minutes. {\ 0042552 aicnsoeoee 2 hours, 36 minutes. 
yy ceteereh senate Immediately. i OOO = cca eh aes _.-| Immediately. 
Boe Se a ees do. Hee bits Ua AleeT — baat 2 ee 5 hours. 
G7h tee ge 5 hours. G25 = ee ee eee 10 minutes. 
idan ae eaten 7 hours. | 6245 322. cea | 7 hours, 37 minutes. 
In some of the animals the symptoms followed immediately after 
the feeding. The longest time before symptoms was 14 hours and 
55 minutes in the case of Sheep 449. This probably is longer than 
the actual time, for the symptoms were first noted in the morning 
and it is probable that the animal had been sick during the night. 
Excluding Sheep 449 the average time elapsing between the feeding 
and the development of symptoms was 2 hours and 5 minutes. On ~ 
the average the symptoms appeared somewhat more quickly than 
was the case with Z. paniculatus. 
RESULT OF AUTOPSYS. 
Opportunity was found for only one autopsy, that on Sheep 599. 
In this animal the left ventricle was contracted and the right expanded. 
The left lung was highly congested, and the right lung congested to 
the point of hepatization. The extreme Poulin of ‘the right lung 
was probably due to hypostasis. No other abnormal conditions 
were noticed. 
TOXIC AND LETHAL DOSAGE. 
Table 6 SHows the dosage of the animals poisoned 1 in 1917, 1920, 
and 1921. 
To determine the pr obable toxic dosage of the plant it is necessary, 
.of course, to consider only the mimimum quantities required to pro- 
duce intoxication or death. 
It should be noted in connection with this cable that although both 
seeds and fruit heads were used, no results were obtained fou the 
dosage used from these parts of the plant. Sheep 630 received 0.807 
pound of seed with no effect, and Sheep 594 received 2.437 pounds 
of fruit heads with no effect. | 
So far as Table 6 is concerned, it appears that in general terms the 
toxic dose of Z. elegans varies from 2 to 4 pounds per 100 pounds of 
animal. 
Lethal dose. —Only one animal, Sheep 599, was killed by Z. elegans. 
The quantity used in this case was 6.153 pounds of leaves, stems, and 
