of Edinburgh, Session 1881-82. 
619 
has slight shiverings. Respirations 30, pulse 104, feeble 
and intermittent temperature 106° Fahr. 
“ Hearing and sight unimpaired. Ears move freely and fre- 
quently. Hose and lips not unusually dry. It attempts 
to nibble grass in neighbourhood, and sprawls with the 
fore legs. Head drawn back. Muscles of extremities 
relaxed. 
“ Four hours after these observations, the left hind leg was 
completely paralysed.” 
The lamb was evidently sinking rapidly, and could not recover. 
Accordingly it was killed, and the post-mortem appearances were as 
follows : — 
“ Several of the ecchymoses in subcutaneous tissue of lumbar 
region (probably because it had been lying for several 
days). Lungs comparatively healthy. Some strongyles. 
Liver showed at one part several grey-coloured nodules 
the size of a split-pea (perhaps small incipient abcesses). 
Kidneys, spleen, and other organs healthy. Small intes- 
tine showed several small punctiform ecchymoses in the 
serous coat. Solitary glands not enlarged. Rumen con- 
tained much undigested grass. Small intestine empty. 
Brain and spinal cord apparently quite healthy.” 
These are the symptoms and post-mortem appearances of what 
seemed an undoubted case of louping-ill. Mr. Scott, Drynoch, put 
his small laboratory at our service, and Mr. Hamilton and I 
remained for some days, in order to determine whether the disease 
was one of an organismal kind. We found in the blood, but 
especially in the cerebro -spinal fluid, a number of exceedingly small 
rounded organisms frequently going in pairs. We endeavoured to 
cultivate these in a weak decoction of mutton. The cerebro-spinal 
fluid was cultivated in drops of the decoction attached to the lower 
surface of thin cover glasses, which were set on rings of putty that 
had been arranged on glass slides, so as to form an efficient live-box, 
Fig. 1. 
as in fig. 1 . Three of these, along with other three containing drops 
of the decoction which had not been inoculated, were put in a 
