1899-1900.] Dr J. S. M‘Kendrick on Enzymes in Tissues. 73 
and any reduction was noted. If there was any re- 
duction, then the probability was that sugar had been 
formed, and the fluid was submitted to further tests. 
To 5 c.c. of the mixture were added 1 decigramme of 
phenyl-hydrazine hydrochloride, and 2 decigrammes of 
sodium acetate. The mixture was heated for half-an- 
hour, and the deposit which formed on cooling was 
examined microscopically for crystals of phenyl- 
glucosazone and phenyl-maltosazone.* 
2. 10 c.c. of X were added to 1 grm. of fresh fibrin in beaker. 
The extract was diluted up to 40 c.c. of cold water. 
The beaker was covered with a glass lid, and placed 
in the incubator for twenty-four hours, at the same 
temperature (38° C.). 
The appearance of the fibrin was noted, and to a 
portion of the filtered fluid was added an equal quantity 
of sulphate of ammonium, and the presence or absence 
of a precipitate was observed. 
3. 10 c.c. of X, diluted up to 40 c.c. with a 0*2 per cent, solution 
of hydrochloric acid, were added to 1 grm. of fibrin in 
beaker. The beaker was covered and placed in in- 
cubator as before. 
The appearance of the fibrin was noted, particular 
attention being paid to see whether there was any 
appearance of digestion. The biuret test was applied 
to the filtered solution, and the presence or absence 
of a rose pink hue observed. 
4. 10 c.c. of X, diluted up to 40 c.c. with a 1 per cent, solution 
of carbonate of soda, were added to 1 grm. of fibrin 
in beaker. The beaker was covered and placed in 
incubator as before. 
The appearance of the fibrin was noted to see whether 
any erosion of it had occurred. A portion of the 
filtered fluid was examined by the biuret reaction, 
while another portion was evaporated down to a few 
* I may state here that on no occasion did I observe the typical crystals 
which occur in sheaths and bundles. I obtained frequently crystals, yellow 
in colour, small, and almost amorphous in character. 
