Arthur: Cultures of Uredineae in 1915 127 
was sown March 31, on Sniilax hispida; another collection from 
the same locality, made May 16, 1915, was sown on another plant 
of the same host May 24, both without infection. The leaves 
of the two collections sent by Mr. Bethel were thickly covered 
with the prominent, blackish telia. The teliospores germinated 
well, especially so in the October collection. Mr. Bethel stated 
on the label of the latter specimen that although the rust was very 
abundant, there were no Smilax plants in the region. In sending 
the May collection he stated on the label that the aecial stage oc- 
curred on Leucocrintim, but desired to have the material tested on 
Smilax. In the accompanying letter of same date he says : “ I 
am inclosing Puccinia amphigena on Calamovilfa longifolia, which 
I wish you would sow on Smilax, which I believe you give as host 
in the east. I have fine young plants of native Smilax, but have 
failed to inoculate them. With us this does not normally belong 
to Smilax, and may be another race. It belongs to Leucocrinum, 
on which it is epidemic everywhere this year.” This species of 
rust was first cultured on Smilax in 1902, from telial material 
gathered at Callaway, Neb., and in the year following from ma- 
terial gathered at the type locality in Chicago, 111 . It has been 
cultured altogether six times,® the westernmost locality for telial 
material 'being central Nebraska, on the plains, some two hundred 
miles from the foothills of the Rocky Mountains, where this 
season’s material was obtained. 
2. Puccinia emaculata Schw., on Paniciim capillare L., col- 
lected at Lafayette, Ind., was sown. May 21, on Euphorbia corol- 
lata, having shown strong germination, but without results. The 
reasons for this attempt, with citation of other trials, are given in 
the previous report of cultures.* 
3. Puccinia simillima Arth., on Phragmites communis Trin., 
collected by Dr. J. F. Brenckle, at Wiedmer’s Lake, N. Dak., on 
April II, 1915, was sown May 14, on Anemone cylindrica and 
Ranunculus acris, without infection. It was sown again June 18 
on two plants of Ranunculus sceleratiis, but with no infection. 
3 See Bot. Gaz. 35:20. 1903; Jour. Myc. 10: ii. 1904; 12:16. 1906; 
14:15. 1908; Mycol. 2:225. 1910; and 4:18. 1912. 
* Mycol. 7 : 65. 1915. 
