Arthur: Cultures of Uredineae in 1915 133 
kind sent the writer by Mr. Bethel was dated Sept. 17, 1910, and 
was intended for cultures on Thalictrum. This could not be 
made to germinate when time for culture work arrived, nor could 
a half dozen other collections sent subsequently at various dates. 
With a specimen dated April 5, 1912, Mr. Bethel states that he 
has “ proven by most positive field cultures ” that it goes to 
Thalictrum Fendleri. Again in May, 1915, Mr. Bethel sent fine 
materia! from his garden, showing the telia that had been brought 
there and the aecia on Thalictrum derived therefrom. The rea- 
sons for listing the Thalictrum form under P. Agropyri were 
stated in the last report.^^ The aecia are especially large and 
prominent in this particular race on Festuca, as shown by a num- 
ber of collections sent from the same locality. 
5. PucciNiA Asperifolii (Pers.) Wettst. — Two plants of An- 
chusa officinalis L. were given ample space in the experimental 
garden of the Indiana Experiment Station and attained a fine de- 
velopment, each plant measuring five or six feet across. On July 
2, the weather being especially damp with occasional light showers, 
stems of rye, heavily infested with rust, were laid upon the two 
plants in the garden, doubtless a hundred or more stems to each 
plant. The plants were not covered in any manner. The date of 
the appearance of the first pycnia was not recorded, but on July 
13, the plants were conspicuously dotted with yellow spots bear- 
ing groups of pycnia. About a week later careful search brought 
to light two groups of aecia. Although the vigor of the plants 
and the condition of the weather seemed favorable, and the great 
numbers of yellow spots with their groups of pycnia continued to 
show, yet no further development of the rust took place.^® 
The reason why the infection should have started out with such 
marked vigor and yet failed to mature aecia is not at all apparent. 
The weather for the whole period was favorable for aecial growth. 
It is possible, and yet scarcely probable, that the explanation lies 
in the lessened vigor for the American rye rust, which is propa- 
gated by repeating spores alone, the aecial hosts being practically 
wanting in America. 
12 Mycol. 7 : 74. 1915. 
1® For a previous culture of similar character see Mycol. i : 236. 1909. 
