240 
Mycologia 
tilled water in test tubes and autoclave at 115° C. for one-half 
hour. 
Eucalyptus twigs. Select twigs of Eucalyptus botryoides about 
% inch in diameter, and by alternate diagonal and transverse 
cuts divide into sections approximately inches long. Place 
with the slanted surface upward iii test tubes containing 2 or 3 
c.c. of distilled water. Autoclave for 15 minutes at 115° C. 
Potato agar. Boil 250 grams of sliced potato in 1000 cc. of 
distilled water for i hour. Filter through cotton. Restore loss 
due to evaporation, etc. Add 115 grams of shredded agar; steam 
until thoroughly dissolved. Filter through cotton. Tube and 
autoclave for 15 minutes at 115° C. 
All cultures were incubated under ordinary laboratory condi- 
tions, the mean temperature during the period being 21.5° C. 
Cultures on Rice. — On this medium the behavior of the fungus 
is character^ized by a rather tardy and scant production of pig- 
ment — a character which readily distinguishes it from the other 
known species of Endothia occurring in North America and the 
West Indies. Within three or four days after inoculation, there 
is a somewhat short cottony growth of mycelium over the upper 
surfaces of the medium. After five to seven days, small points 
of orange-yellow appear in places where the pycnidia are begin- 
ning to form and the older aerial mycelium changes to ivory- 
white or pale-buff. In about fifteen days, the rather large yellow 
pycnidia mature and exude moist masses or tendrils of bright- 
yellow or orange-colored spores. No marked submerged color 
changes of any kind take place, the medium merely turning to a 
pale-dirty-buff as growth proceeds. The aerial mycelium re- 
mains white or pale-buff and the yellow color is seen only where 
the pycnidia have developed. No further changes occur except 
that in old cultures pycnidia formed against the sides of the 
tubes often become dark-brown. 
Cultures on Eucalyptus Twigs. — Cultures five days old show a 
thin web-like growth over the cut surfaces, with here and there 
small masses of yellowish mycelium where pycnidia are begin- 
ning to form. Two days later, a few pycnidia mature and the 
exudation of spore masses begins. Pycnidia continue to form on 
the cut surfaces and later on the bark. The surface growth re- 
