288 
Mycologia 
Elongated and irregular forms are also produced from somewhat 
swollen and knotted segments of the hyphae. 
Under ordinary cultural conditions such as on flies, ant larvae, 
gnats, mushroom grubs, etc., in water there is usually no sexual 
reproduction. Out of a hundred cultures perhaps one would 
show a few oogonia. A number of experiments have been made 
to induce the formation of oogonia. The results of some of these 
are as follows : 
On a bit of whole egg agar in distilled water: Growth vigorous and healthy. 
Sporangia abundant, emptying normally and proliferating laterally from 
below. No oogonia or chlamydospores. 
On a bit of whole egg agar in distilled water : Growth vigorous, reaching a 
diameter of 4 cm. Sporangia slow to form, but after full growth appear- 
ing rather plentifully. Chlamydospores of usual shape present, but not 
plentiful. No oogonia. 
On a bit of hard boiled egg yolk in distilled water : Growth vigorous, reaching 
a diameter of 4 cm. Sporangia abundant and formed earlier than in 
culture above. Chlamydospores plentiful. Oogonia present, but scarce. 
Antheridia of diclinous origin. As this was a culture from a single spore, 
the presence of antheridia proves that the plant is not dioecious. 
On a bit of whole egg agar in spring water: Growth vigorous and strong. 
Many chlamydospores. No sexual reproduction. 
On fly in spring water : Growth vigorous. Many sporangia, all proliferating 
from side below as in Achlya. No chlamydospores or oogonia. 
On corn meal agar : Growth extensive, filling dish. Aerial branches nearly 
reaching cover, but not dense. Only chlamydospores present. 
In 5 per cent, maltose + 0.1 per cent, peptone solutions mixed half and half: 
Growth vigorous and healthy. A few small sporangia were formed, but 
the spores were only imperfectly discharged. Also a few of the charac- 
teristic knob-like chlamydospores. 
On corn meal agar in tightly stopped sterile bottle : Growth vigorous, extend- 
ing across bottle and making a mold-like aerial growth an inch high. On 
examination there were found only single chlamydospores, most of which 
were quite empty, they having sprouted by a slender thread about 3 fi 
in diameter. In fact all the growth was remarkably slender (3 /i in 
diameter), enlarging to normal size only just below the chlamydospores. 
On corn meal and egg yolk agar : Growth very strong, covering dish and devel- 
oping abundant aerial hyphae that reach the cover. No reproduction of 
any kind. 
The following six cultures were all made on ant larvae in dis- 
tilled water with the salt added as indicated: 
In 0.1 per cent. KN 0 3 : Growth good. Normal sporangia, discharging and 
spores taking second swim. Many good chlamydospores of usual shape, 
the larger ones having a tendency to form the cross wall some way up 
from the base. No sexual reproduction. 
