Coker: Two New Species of Water Molds 
289 
In o.i per cent. KH 2 P0 4 : Growth good. Many normal sporangia discharging, 
and spores taking second swim. Many chlamydospores. No sexual re- 
production. 
In o.i per cent. Na 2 HP0 4 : Growth good. Many normal sporangia discharg- 
ing, and spores taking second swim. A good many chlamydospores, but 
not so numerous as in the preceding cultures. No sexual reproduction. 
In o.i per cent. K 2 S0 4 : Growth slight. Culture infested with fungus. Spo- 
rangia formed but not discharging. A few chlamydospores. No sexual 
reproduction. 
In o.i per cent. Ca 3 (P0 4 ) 2 : Growth good. Many normal sporangia discharg- 
ing, and spores taking second swim. Many chlamydospores of usual shape. 
No sexual reproduction. 
In o.i per cent. Ca(N0 3 ) 2 : Growth good. Many normal sporangia discharging, 
and spores taking second swim. Many chlamydospores. No sexual re- 
production. 
The following seven cultures were all made on hard boiled egg 
yolk in distilled water with the chemical added as indicated : 
In o.i per cent. KN0 3 : Strong growth. No sporangia. A few good chlamy- 
dospore. No sexual reproduction. 
In o.i per cent KH 2 P0 4 : Growth good. A very few sporangia with normal 
discharge. No chlamydospores or sexual reproduction. 
In o.i per cent. Na 2 HP0 4 : Strong growth. Abundant sporangia proliferating 
repeatedly, and discharging normally. A very few chlamydospores. No 
sexual reproduction. 
In o.i per cent. K 2 S0 4 : Strong growth. Sporangia plentiful. Chlamydospores 
abundant. No sexual reproduction. One sporangia was seen discharging. 
The emergence was rather slow, and the last few spores were very 
slow and showed obvious swimming movements in the sporangium on 
escaping. About a dozen clung to the tip of the sporangium. The others 
spread in a loose flock, showing slow movements, and every now and then 
one would swim briskly away. 
In o.i per cent. Ca 3 (P0 4 ) 2 : Strong growth. Many sporangia, quite normal. 
A very few chlamydospores. No sexual reproduction. Several sporangia 
seen to discharge. Six spores detached themselves at different points and 
moved away, soon stopped and settled to the bottom. All others remained 
attached in a pretty solid mass to the tip of the sporangium. In another 
case four were detached. In another case six were detached. 
In o.i per cent Ca(N0 3 ) 2 : Very little growth. A good many chlamydospores. 
Nothing else. 
In o.i per cent. K 3 P0 4 : Strong growth. Abundant sporangia. Many good 
chlamydospores that look exactly like oogonia initials, and a good many 
smaller branches that suggest antheridial branches, but no oogonia. 
The genus Pythiopsis has until now included but one species, 
P. cymosa, discovered in Germany by De Bary . 4 It has been 
recognized only one since, it seems, when Humphrey 5 found it 
* Bot. Zeit. 46 : 63. 1888. 
s The Saprolegniaceae of the United States. Transactions Amer. Philos. 
Soc. 17 : part 3. 
