292 
Mycologia 
Resting bodies of more or less globular shape are formed in 
quantity and are often arranged in chains (fig. n). After a rest 
these also form zoospores. 
Our new species of Pythiopsis has appeared eight times in col- 
lections made in the neighborhood of Chapel Hill. It was first 
obtained on Feb. 29th, 1912, from collections made at intervals 
along the brook that flows from the spring about 100 yards to the 
northwest of Dr. Archibald Henderson’s residence. A tumbler 
of water was taken at each place with a little mud and any algae 
and dead leaves, twigs, etc., that happened to be present. Ant 
larvae were floated on the surface of the water of each tumbler 
and in four of these appeared a species of Pythiopsis that was 
found to be new. It was also found on the same day in a springy 
marsh on the south side of Glen Burnie Meadow and appeared 
subsequently in the brook in Battle’s Park (March 18, 1912), in 
the branch south of the South Building, U. N. C. (March 25, 
1912), and again in the Glen Burnie Meadow Marsh (May 13, 
1912). The plant was separated from other fungi present and 
was grown in pure cultures for about six months. I have named 
the species in honor of the late Dr. James Ellis Humphrey, author 
of “ The Saprolegniaceae of the United States,” whose work has 
been of great assistance to all students of this group in America. 
The species may be defined as follows : 
Pythiopsis Humphreyana sp. nov. 
Vegetative growth of long, slender, sparingly branched hyphae 
of about 11 to 14 ju. in diameter throughout, stouter in the neigh- 
borhood of the reproductive bodies, after maturity disorganizing 
rather quickly; sporangia varying in shape from spherical, oval 
or pyriform to elongated, tapering and irregular forms, discharg- 
ing by a short or rather long papilla and usually proliferating 
from below in a cymose manner ; spores monoplanetic, pear-shaped 
and with two cilia, about 8.9 /u, in diameter on coming to rest; 
oogonia generally borne exactly like the sporangia and not to be 
distinguished from these when young, apical and often in groups 
by cymose branching, usually spherical with a basal neck, some- 
times pear-shaped and rarely longer and more irregular, varying 
greatly in size, diameter from 33 to 89 fi, averaging about 43 /*; 
wall always smooth and unpitted, about 1.4 /a thick; oospores 
generally one, occasionally two and very rarely four, centric, 
