G34 
Louping 111. 
other culture tube (there were in each case at least three gelatine 
and three agar cultures made), one colony of a different species of 
bacteria was obtained. But these other species were not the same 
in any two cases, and owing, therefore, to their scarcity and to their 
exceptional appearance, they may be at once dismissed as accidental 
contaminations ; that is to say, as introduced in the process of 
preparing the cultivations. But the above species that occurred in 
the six cases was uniformly present, and, besides, was in the case 
of two of the animals very abundantly present in each culture 
tube. 
The colonies of this species are round, somewhat raised above 
the surface of the nutritive medium. They appear only slowly on 
gelatine, being three to four days before they are sufficiently 
developed to be seen with the naked eye ; but on agar incubated 
at 35-37° C. they are noticeable by the second or third day. 
The colonies are of a distinctly yellowish aspect when looked at in 
reflected light, but when seen in transmitted light are brown. 
In streak- culture they form a yellowish band-like growth in the 
line of the streak, which at first is greyish and narrow, gradually 
as growth proceeds becoming broader and of a yellowish colour. 
The outline is knobbed or crenated, thinner at the margin, thicker 
in the middle. When this band has been growing for some time — 
one to two weeks — on gelatine, it looks moist and markedly yellow 
in the thicker parts. Later on the edge becomes filmy and slightly 
corrugated. In stab-culture the line of the stab becomes marked 
as a linear aggregation of greyish round droplets, brown in trans- 
mitted light. On the surface of the stab there is, after some days, 
a slightly prominent yellow knob ; at first very limited, but gradually 
spreading out into a patch-like yellow mass. The gelatine is not 
liquefied. Milk is not coagulated by the growth of the microbe. 
Faintly alkaline broth becomes, by the growth of this organism, 
turbid in a couple of days ; later the broth shows a fairly copious 
floccular greyish precipitate. 
Examined under the microscope, the growth is composed of 
minute non-motile rods or bacilli ; short ovals to fairly long rods, 
measuring on the average 0'6-0'8-1/x 1 in length, 0’2-0 - 3 p. in 
thickness. As a rule the ovals and rods form dumb-bells ; the 
individual elements are sometimes almost spherical, in most 
instances, however, distinctly ovoid. I have tried inoculation with 
material from cultures of these bacilli on rabbits, guinea-pigs, and 
mice. The result of these is as follows : — Injecting a fair dose of 
the growth (gelatine or broth cultivation) into the subcutaneous 
tissue, a swelling is produced which, after 48-72 hours, becomes 
very marked ; then it diminishes, becoming at the same time firm. 
Constitutionally no abnormal symptom is produced ; the animals 
remain lively and continue to feed well. This practically negative 
result cannot, however, be accepted as indicating that the bacillus 
