6 
Mycologia 
wood extract, prune decoction and a solution of beef extract and 
malt.® The best germination was obtained with chestnut wood 
extract. Slight germination occurred in tap water and in the 
malt beef extract ; the others gave negative results. 
Before germination, or with its inception, the spore becomes 
vacuolate (Figs b and e). Then germination occurs at one or 
both ends. Lateral germ tubes were not observed. A small 
papilla, always of smaller diameter than the spore, is formed, 
which gradually elongates to form the germ tube. At the end of 
forty-eight hours, some of the germ tubes attained a length of 
from four to six times the length of the spore (Fig. 8). There- 
after growth was very slight. In preparations a week old, here 
and there a germ tube had branched, but in general only slight 
elongation occurred after the second or third day. Mycelial 
wefts were not obtained in hanging drop cultures. Rarely one or 
two cross walls were formed in the germ tube, but these were 
never accompanied by clamp connections. 
An earnest effort to secure pure cultures of the fungus led to 
no tangible results. Various media were tried, among which may 
be enumerated prune agar, bean agar, chestnut wood decoction 
agar, chestnut wood decoction gelatin, corn meal moistened with 
wood decoction, corn meal moistened with tap water, sterilized 
chestnut wood, sterilized oak wood, bean tubes, potato tubes, 
carrot tubes, and a medium made after Marpmann’s formula.^ 
Inoculations were made with newly fallen spores and with germ- 
inating spores from hanging drop cultures. A sparse, floccose 
mycelium from germinating spore infection was formed in the 
bean tubes, but the hj’phae were always restricted in their growth. 
Attempts to transfer these colonies to more favorable media re- 
sulted in failure. New experiments are now under way with 
this object in view, and it is to be hoped that they may be more 
fruitful of results. The timber rot accompanying the fruit bodies 
3 zYi gm. Liebig’s beef extracti®, 2 j 4 gm. Lofflund’s malt extract, loo c.c. 
water. 
^ Dissolve by cooking lo gm. of gelatin and lo gm. of agar in 500 gm. 
of beef extract; then add to the solution 10 gm. glycerine, 10 gm. salt, 5 gm. 
ammonium phosphate, and 4 gm. potassium nitrate ; filter. 
