Arthur: Cultures of Uredineae 
65 
sown April 17 on Lithospermiim officinale^ Aesculus glabra, Ele- 
agnus argentea, Lepargyraea canadensis, and Doellingeria nmbel- 
lata. It was sown again March 20 on Sphaeralcea lobata, Calli- 
rhoe digitata and C. involucrata. No infection was secured. 
This is the first attempted culture of this species with telio- 
spores, although once grown from amphispores.® Most collec- 
tions show only the amphisporic stage of the rust. No clue to the 
alternate host has yet been obtained. 
3. PucciNiA EMACULATA Schw. — This exceedingly common 
rust on Panicum capillare has been used in previous years for 
sowings on 26 different species of hosts with no infection.* A 
careful morphological study shows that the species in its uredinial 
and telial conditions is very like Puccinia Pammelii Arth. (P. 
Panici Diet.), occurring commonly on Panicum virgatum, whose 
aecia have been grown upon Euphorbia corollata. Since this fact 
was ascertained, attempts have been made to grow P. emaculata 
upon Euphorbia corollata. Telial material from the vicinity of 
Lafayette, Ind., was sown accordingly on April 28, 1913, March 
24, 31, and April 6, 1914. Material collected by Dr. Fromme at 
Lakehurst, N. J., was sown May 8, 1914, on both E. corollata and 
E. cyparissias. A collection from Lafayette was also sown March 
31, 1914, on Chelone glabra, and Dirca palustris, which had not 
previously been tried. All attempts were equally fruitless. It is 
hoped that some time a fortunate field observation may lead to 
the solution of the problem. 
4. Uromyces Rhyncosporae Ellis. — No satisfactory field 
clues to the alternate host of this species have yet been obtained. 
Morphological study has led to the suggestion, improbable as it 
may seem at first, that it can belong to the very common Carex- 
Aster-Solidago complex. To test this suggestion, material ob- 
tained by Dr. Kern and the writer at Auburn, Ala., was sown 
April 22, 1912, on Aster paniculatus, Solidago canadensis, and 
Helianthus angiistifolius. Another set of sowings was made on 
May 8 following upon other plants of the same three hosts, and 
on Ruellia strepens, and Apocynum cannabinum. No infection 
3 Jour. Myc. 14: 20. 1908. 
^ Bot. Gaz. 35: 12. 1903; Jour. Myc. 8: 52. 1902; 10: 10. 1904; 12: 12. 
1906; 13: 192. 1907; 14: II. 1908; and Mycol. i: 230. 1909. 
