72 
Mycologia 
years^^ to culture this rust, thirty-five species of hosts having 
been used other than those used this year. Only once was a plant 
of Viola used, V. striata, a caulescent species on which such 
aecia have not yet been found, and are not very likely to occur. 
The successful cultures were inspired by field observations by 
Dr. Brenckle, who sent us material with which to test his predic- 
tion. In addition to Dr. Brenckle’s opinion there was also at hand 
the opinion of Mr. C. R. Orton, at that time working on the rusts 
in my laboratory, which was drawn from a study of correlation 
between this species and Uromyces pedatatus, in a paper read 
December 28, 1911, but not published until later,^® the latter spe- 
cies having its aecia on acaulescent violets. Since that time Mr. 
W. H. Long has published^® extended studies, both cultural and 
morphological, with important bearings which can not be taken 
into consideration in this connection. 
5. PucciNiA Stipae Arth. — Two collections with teliospores on 
Stipa comata, gathered by Mr. E. Bethel, from different spots at 
Boulder, Colo., were used for cultures. One was sown May 15 
on Sene do lugens, S. spartioides,^’’ Chrysopsis villosus, Grindelia 
squarrosa, Gntierreda Sarothrae, and Solidago mollis, all consid- 
ered probable hosts for the aecia. Infection occurred only on 
Gutierrezia Sarothrae, showing pycnia May 22, and aecia May 27, 
both in abundance. 
The other collection was sown ]\Iay 17 on the same set of hosts 
with infection only on Senecio spartioides, showing numerous 
pycnia May 23, and aecia June i. 
This species has previously been cultured^® on Aster multiflorns, 
A. ericoides, A. Novae-Angliae, Solidago canadensis, Grindelia 
squarrosa, and Senecio lugens. The present study adds two 
See Jour. Myc. 14: 10. 1908; Mycol. i; 231. 1909; 2; 220. 1910; and 
4: 9. 1912. 
15 Mycol. 4: 199, 200. pi. /O, figs. 5 and 6. 1912. 
1® Notes on three species of rusts on Andropogon, Phytopath. 2; 164-171. 
August, 1912; and Influence of the host on the morphological characters of 
Puccinia Ellisiana and Puccinia Andropogonis, Jour. Agric. Research 2: 303- 
319. July, 1914. 
11 The same host that was erroneously called S'. Douglasii in report for 
1910. Mycol. 4: 9, II. 1912. 
18 See Jour. Myc. 11: 63. 1905; and Mycol. 4: 19. 1912. 
