Arthur : Cultures of Uredineae 
73 
aecial hosts to the list previously known, and further strengthens 
the view that the species consists of a number of loosely defined 
races. 
6. PucciNiA Agropyri E. & E. — Three collections on Elymiis 
canadensis from two widely separated and very different habitats 
were successfully cultured. One collection made by Mr. E. 
Bethel at Boulder, Colo., from plants on which he had previously 
placed leaves of Clematis ligusticifolia covered with aecia, 
brought from thirty miles distant, was sown April 27, on Cle- 
matis ligusticifolia, C. Douglasii, Anemone cyiindrica, Delphinium 
Geyeri, and Thalictrum Fendleri, all believed to be probable hosts 
of this rust, and on Phacelia heterophylla, Hydrophyllum capi- 
tatum, H . Fendleri, and Onosmodium occidentale, which have been 
suspected from field observations to belong to the list of hosts. 
Infection only occurred on Clematis ligusticifolia, showing pycnia 
May 8, and aecia May 20. 
Another collection by Mr. Bethel from plants growing with 
aecia-bearing Clematis lingusticifolia, at Boulder, Colo., was 
sown May 14, on the same nine species of hosts, with infection 
only on Clematis ligusticifolia, showing pycnia May 21, and 
aecia May 29. 
The third collection by Dr. Brenckle from Kulm, N. Dak., was 
sown on Clematis ligusticifolia, C. Douglasii, C. virginiana, Ane- 
mone cyiindrica. Delphinium sp. from Colorado, Thalictrum Fend- 
leri, T. alpinum, Aquilegia caerulea, and A. flavescens, all belong- 
ing to a quite possible group of hosts, and on the outlying 
hosts, Phacelia heterophylla, Hydrophyllum Fendleri, Onosmod- 
ium occidentale, and Flaeagnus argentea. Infection was obtained 
only on Anemone cyiindrica, showing a few pycnia ]\Iay 20, but 
without maturing aecia. 
Another collection of the same rust on Agropyron Smithii 
(host determined by Prof. A. S. Hitchcock), collected by the 
writer at Pueblo, Colo., was sown April 29, on Clematis ligustici- 
folia, C. Douglasii, Anemone cyiindrica. Delphinium sp. from Col- 
orado, Thalictrum Fendleri, Hydrophyllum capitatum, H. Fend- 
leri, and Onosmodium occidentale, with infection only on the first 
named. The pycnia began to show May 8 and aecia May 17. 
