82 
Mycologia 
made of the same material on S> lobata, C. digit ata, Hibiscus 
mUitaris, and Malvastrmn coccineum, with infection only on the 
Sphaeralcea, pycnia showing April 6, but the aecia being destroyed 
by aphis before maturing. 
Another collection of identical appearance was made five days 
later than the one above, at Ysleta, Texas, a few miles from El 
Paso. This was sown, March 28, on two plants of 5 ". lobata, on 
C. digitata, and H. militaris. Both plants of Sphaeralcea gave in- 
fection, showing pycnia April 6, but resulting in few aecia, owing 
to accident. Another sowing from the same collection was made, 
May 2, on Napaea dioica, without infection. 
Putting the above cultural results from P. tosta, with the gross 
appearance and subsequent microscopic study, and comparing with 
similar data from P. Muhlenbergiae, there appears to be no 
ground upon which to keep the two forms separate. The fact that 
the telial hosts belong to two genera of grasses is not in this case 
a difference of importance, as Sporobolus and Muhlenbergia in- 
tergrade, and are kept separate upon technical grounds. The 
previous cultural studies®® of P. Muhlenbergiae have indicated 
that the species is a complex of races. It has been grown on 
Hibiscus militaris and Callirhoe involucrata, but the similar aecia 
on Napaea, Malvastrum, and Sidalcea have not yet been grown, 
although attempted, the failure believed to be due to a lack of the 
proper racial telia. The above is the first successful culture with 
material on Sporobolus, although previous attempts®® have been 
made. 
Hereafter it will be considered advisable to treat P. tosta 
as a synonym of P. Muhlenbergiae. 
21. Puccini A Agropyri E. & E. — A collection of this rust on 
Elymus virginicus was made by Dr. Fromme and the writer on 
Feb. 13, 1914, at Austin, Texas, and at the same time the observa- 
tion was made that Clematis Drummondii, not then in leaf, was 
in the same spot, and abundant in the vicinity. Dormant roots 
of this wild Clematis were secured, and later grown for the culture 
work. 
35 See Jour. Myc. 11; 51. 1905; 13: 192. 1907; Mycol. i: 251. 1909; 2: 
226. 1910; and 4 : 18. 1912. 
3 ® See Jour. Myc. 10: 10. 1904; 12: 12. 1906; Mycol. 4: 10 and 52. 
1912. 
