Arthur ; Cultures of Uredineae 
85 
Cultures in 1913 
1. Uromyces elegans (B. & C.) Lagerh. — This autoecious 
rust, very common in the Southern States, is one of the group 
of species which produce no uredinia, so far as known, very few 
of which have been cultivated. Growing plants of Trifolium car- 
olinianum bearing aecia were sent by Dr. F. A. Wolf from 
Auburn, Ala., and the aeciospores sown March 29 on plants free 
from the fungus. Teliospores began to appear about April 18 
as the result. 
This result does not disclose whether the species possesses both 
primary and secondary aecia, or whether pycnia ever occur. A 
culture with teliospores should be kept in view. 
Cultures in 1914 
2. PucciNiA NODOSA Ell. & Hark. — While upon a visit to the 
Desert Botanical Laboratory at Tucson, Ariz., an exceptional op- 
portunity was offered to study the life history of another autoe- 
cious species of rust, which like the one last mentioned, possesses 
no uredinia. A strong plant of Brodiaca pauciflora, growing near 
the door of the laboratory was moistened on February 26, sprinkled 
with aeciospores brought from some distance, and covered with a 
helljar. The temperature at the time was favorable for infection, 
the helljar being shielded from the direct rays of the sun. The 
experiment was left in charge of Dr. W. A. Cannon of the Labo- 
ratory staff, who kindly forwarded the leaves on March 18, when 
the sori first opened, (ffily telia were produced. 
As in the preceding case the possibility of both primary and 
secondary aecia occurring in the life cycle is not touched upon. 
3. PucciNiA SPLENDENS Vize. — Through the courtesy of the 
Desert Botanical Laboratory, Dr. Fromme and the writer were 
enabled to make an excursion on February 28 to the Santa Rita 
Mountains in the vicinity of Tucson, Ariz., where we secured 
teliosporic specimens of P. splcndens on Hymenoclea nionogyra. 
Through the kindness of Prof. J. J. Thornber of the University 
of Arizona we received after our return to Indiana a number of 
thrifty young plants of this host, which soon started into leaf in 
the green house. A sowing of teliospores was made April 7, 
