148 
Mycologia 
was impossible to determine the genus with any degree of cer- 
tainty. 
17. Phyllosticta bixina sp. nov. 
Spots yellowish-brown, scattered, subcircular to irregular, 5- 
10 mm. in diam., numerous, amphigenous, lighter below, often 
bordered by an intermediate yellow region which spreads over 
the leaf, older portions of the spot bordered by a distinct brown- 
ish-black margin ; pycnidia few, epiphyllous, spherical, dark- 
brown, 92-148/4 in diam., ostiole distinct, dark-bordered, 12-24/4 
in diam. ; conidia hyaline, ovate, 4.8-6 X 2.4 /4. 
On leaves of Bixa orellana L. in Porto Rico: Maricao, 174 
(type) ; San German, 5/94; Rosario, 4844; Mayagiiez, 298; 
Coamo, 55; Punta Santiago, 2458; Ahasco, 3208; Adjuntas, 
4975a- 
18. Phyllosticta eupatoricola Kab. & Bub. Hedwigia 46: 
288. 1907 
On Eiipatorinm odoratum L. in Porto Rico: Villa Alba, 103. 
19. Phyllosticta Eugeniae sp. nov. 
Spots amphigenous, circular, 2-4 mm. in diam., somewhat 
darker above than below, the center dark-brown surrounded by a 
lighter colored ring which is bordered by an even, dark, slightly 
elevated margin, centers falling out with age, leaf around the 
spot reddish-brown ; pycnidia more numerous on the upper sur- 
face, dark-brown, spherical to ovate, 84-144/4 in diam.; conidia 
ovate, hyaline, granular, often remaining attached to their stalks, 
9.6-16.8 X 4.8-6 / 4 . 
On leaves of Eugenia buxifolia (Sw.) Willd. in Porto Rico: 
Mona Island, 6230 (type), 6091, and 612'/. 
An ascomycete is associated with this species. Perithecia 
amphigenous, dark-brown to black, spherical, regular, dark-bor- 
dered, 168-228/4 in diam., ostiole often opening laterally; spores 
hyaline, immature. 
20. Phyllosticta araliana sp. nov. 
Spots amphigenous, yellowish-brown, circular to ovate, 4-8 
mm. in diam., bordered by a dark-brown, regular margin; pyc- 
nidia scattered, epiphyllous, spherical, 24-48/4 in diam., dark- 
