Clark axd Smith : Toxicological Studies 
229 
which was relieved in a few seconds by atropin. See Plate 91, 
fig. 2. 
Experiments zcith the Ash of an Active Unpurified Inocybe 
Extract 
Experiment 21. — i\Iay 29. About 15 c.c. of the extract used 
in Exp. 10 were evaporated to dryness and carefully ashed at low 
red heat, the ash was dissolved in water and tested on the heart 
of a frog weighing 29 gm. This solution did not produce the 
slightest flutter in the nonnal tracing and so it is evident that the 
inorganic or ash constituents of the fungus are not responsible 
for its toxic action. 
Experiments zoith Unpurified Extracts of Amanita muscaria 
Experiment p. — i\Iay 22. The action of an Amanita extract 
was tested upon the heart of a frog weighing 33 gm. In this 
case a standstill of the heart was not caused although the usual 
retarding effect was noticed. This effect was partially neutralized 
by atropin. 
Experiment ii. — INIay 23. Experiment 9 was repeated with a 
frog weighing 29 gm. The same slight muscarin effect appeared. 
Experiments zvith Purified Extracts of Amanita muscaria 
Experiment 26. — June 3. The action of a purified extract of 
this Amanita on a frog (weight 31 gm.) was similar to that re- 
corded in Experiments 9 and ll and it did not prove to be nearly 
as toxic as extracts of Inocybe infida or Clitocybe illudens. 
Experiments zvith Unpurified Extracts of Clitocybe illudens 
Experiment 5. — April 16. Frog weighed 35 gm. The heart 
was soon brought to a standstill but was started at once by 
atropin. The action was exactly the same as that of Inocybe 
infida but it did not take place as rapidly. See Plate 91, fig. 3. 
Experiment 14. — May 23. Turtle weighed 633 gm. The tur- 
tle’s isolated heart reacted to Clitocybe extract in a way difficult 
to distinguish from the response to the Inocybe extract. 
