230 
^Mycologia 
Experiments zvith Purified Clitocybe illudens Extracts 
Experiment jj. — ^June 6. Frog weighed 28 gm. The action 
of this purified Clitocybe preparation was the same as that of the 
unpurified extracts. 
» Experiments zvitli the Harmless Clitocybe multiceps Extracts 
Experiment 20. — May 29. As a control on our processes we 
tested the unpurified extract of this fungus on the heart of a 
frog (weight 33 gm.). It was found to be entirely without 
action. Evidently we had not been producing toxic effects 
through our procedures. 
Toxicological Experiments on Frogs 
In order to study the effect of the various fungi upon animal 
organisms as a whole we made injections of the different prepara- 
tions into the dorsal lymph-sacs of frogs. The extracts were por- 
tions of those that were used in the exposed heart experiments ; 
their preparation has already been described. Typical experi- 
ments with the different mushroom extracts will be given in order 
to show the characteristic action of each species. 
Experiments with Purified and Unpurified Extracts of 
Amanita muse aria 
Experiment 50. June 3, 1912. Frog ii weighed 24 gm. Re- 
ceived an injection of i c.c. of purified extract at 5.47 P.M. 
5.49 P.M. Partly paralyzed. 
5.52 P.M. Wholly paralyzed. 
5.56 P.M. Heart stopped. 
5.58 P.M. Received injection of i c.c. atropin solution. 
6.03 P.M. Heart begins to beat weakly and irregularly. 
June 4, 9.15 A.M. Frog ii found dead. 
The results of parallel experiments with unpurified Amanita 
extracts differed in no way from those indicated above. 
Experiments zvith Purified Extracts of Inocybe infida 
Experiment 28. — ^June 3. Frog 8 weighed 23 gm. Received 
injection of i c.c. of this extract at 3.46 P.M. 
3.48 P.M. Partly paralyzed. 
