Agricultural lixpcriineut Station Ueaearch UuUetia H 
A previous test had been made with soy beans, between 
Septeinl^er 20 and December 1, 1010, in an attempt to determine 
whether the presence of nematodes increases the number of in- 
fections. The nematodes were introduced into the soil of large 
buried ])ots in root galls from living soy beans free from infection 
by F usarium spp. The results are presented in Table 8. 
At Lincoln, on December 4, 1917, six stone jars of five gallons 
capacity were filled with nematode infested soil from a greenhouse 
bench in which badly nematode-infected tomatoes were growing. 
Four other jars Avere filled Avith nematode-free potting soil. Cul- 
tures of F usarium groAving on pieces of SAveet cloA’er (Melilotus 
alba) stems, from tAvo 1 liter flasks, Avere incorporated into the 
soil of each jar. Three Clay coAvj^eas and three ^lammoth YelloAv 
soy beans Avere groAvn to maturity in each jar in a greenhouse 
kept at approximately 28° C. during the da}" and 22° C. at night. 
On February IG, Avhen the plants had nearly matured seed pods, 
they Avere remoA^ed and examined. Table 9 shoAvs the number of 
plants affected Avith nematodes and Avith F usarium. 
4Ablk 9. — Infuence of nematodes on infection h\j 
F. trachei phi linn. 
Jar 
No. 
Organisms 
Total r 
of p] 
lumber 
lants 
Per cent affected 
with nematodes 
Per cent affected 
with Fusarium 
1 
jSoy bean 
Cow pea 
Soy bean 
1 
1 r- 
Cowpea 
1 
Soy bean 
1 Cowpea 
[- - 
1 
F usarium and 
nematodes . . . 
3 
3 
1 
100 1 
1 
1 100 
« 
j 100 
2 
do 
3 
3 
100 ‘ 
' 100 
0 
1 100 
3 
do 
3 
3 
100 
100 
0 
100 
4 
do 
3 
3 
100 
■■ 1 
100 
1 
0 
100 
5 
I do 
1 
3 
3 
100 ' 
' 100 
0 
100 
~6~' 
do 
3 
3 
100 
100 
0 
100 
7 
1 Fusarium 
3 
3 
0 
0 
0 
33 s 
8 
do 
3 
3 
0 
0 
0 
1 
! 66 1 
1 
<) 
do 
3 
3 
0 
0 
0 
661 
10 
do 
3 
3 
0 
0 
0 i 
i 
331 
