2() A(frirnJtnr(il h\i‘i>crime)if Station licscitrrji Ballet in IJ 4 
EXPKRIMKXT I. — Tw(‘iity-fi\ e XOrtli Carolina Black cow- 
jx*a and 'Ja Maniniolli \(*llow soy hcan seedlings, growing in each 
of two flats in the greenhouse, were each inoculated when from 
10 to 15 cm. high with sj)ores from sporodochia and with 
mycelium by introducing the material into incisions in the stems 
at 'J to 4 cm. below the surface of the soil. All of the plants in 
()ii(‘ flat Avere inoculated Avith the soy l>ean strain of Fasaiiam 
and all of those in the other AAuth the coAvj^ea strain. Checks and 
all inoculated plants except tAA’o coAvpeas inoculated Avith the soy 
bean strain and one Avith the coAvpea strain remained healthy. 
4'he test Avas repeated, using freshly isolated .^trains of both 
organisms; and, since all but one of tlie ])lants remained healthy, 
this method of inoculation Avas discarded. 
EXPERIMENT IT. — In this experiment the soil in two flats, 
A and B, in the greenhouse AA’as inoculated Avith pure cultures of 
Famriatn s])p. on coAvpea and soy bean, respectiA’ely. These cul- 
tures Avere then incorporated in the ujAper 4 inches of soil. 
In this and succeeding exj)erinients the organisms had been 
groAvn on ])ieces of moistened, sterilized coAvpea stems until 
numerous sporodochia had formed. On AjAril 1*2, IDlb, 20 North 
Carolina Black coAvi)eas and 20 Mammoth YcHoaa’ soy beans Avere 
])lanted in each flat. A third flat, containing uninoculated soil, 
Avas ])lanted as a check. 
By ,June 4 a coAvpea plant on Elat B Avas noted to be diseased. 
Others had been observed to be affected by June 15, Avhen all the 
])lants Avere remoA'ed and examined. The results are presented in 
Table J. 
Taiu.k J. — licsalts of (jroa'iiK! soy heans and coa'j>eas in artifirialtf/ 
inoeo dated soil. 
1 
1 “l 
1 
1 
' Total 
Diseased plants 
Flat i Organism 
i 
Host 
; number ; 
No. 
Percentage 
i 
|of plants 
1 t 
A 1 F. tracheiphilum on cowrea. . ; 
Cow peas. . . . 
— — 1 
20 
6 
30 
1 
- -1 - -1 
Soy beans. . . 
1 
3 
15 
B 1 Fusarium sp. on soy bean. . . 1 
Cowpeas. . . . 
20 
10 
50 
Soy beans. . . 
20 
7 
35 
C None (control) , 
C owpeas. . . . 
20 
0 
0 
Soy beans. . . 
20 
0 
0 
EX PER I .M lON'r III (Scri(‘s 1). — Since (he j)ercen(age of 
dis(‘as(‘d j)ljiu(s in I^k\j)erimenl II is relatively small, the t(*sl Avas 
r(*peat(*(l. using anotluM* strain of I'ach organism and C’lay coav- 
