Agflcultnral Experiment Station Researeh Bulletin H 
accoiiiplislied by transferring a small portion of mycelium to a 
variety of media until a medium was found on which spores 
were again ol)tained. 
All cultures were kept in the laboratory at room temperature, 
12° to 2()° C., and in diffused daylight, so that they were sub- 
jected alike to any change of environmental conditions. 
In all cases 10 cultures of a species were made on each 
medium. Different forms of fructification which normally appear 
on a certain medium may not do so in every tube. For example, 
in a species in which sporodochia are not abundant, they may 
perha])S form on only 2 or of the 10 stem plugs; or if the form 
produces green sclerotia, they may develop on not more than 5 of 
the 10 potato plugs. In some instances as many as 8 to 10 sets of 
10 tubes each of a ])articular species were made. 
In making the microscopical examination, note was taken of 
the size, septation, abundance, and type of conidia (fig. 1, A-G), 
chlamydospores, and conidiophores. In measuring spores, several 
fields were first examined to fix in mind the prevailing type and 
an av(‘rage of 10 or more of these typical spores was made. Care- 
ful note was taken also of extreme types. 
In the macroseo])ic studv of the cultures, the nature of the 
stromata, the pionnotes and sporodochia, the character of the 
aei'ial mycelium, the color of spore masses, aerial and submerged 
mycelium, and substratum, and the production of sclerotia were 
considered. 
RESULTS OF THE COMPARISON OF THE SOY BEAN FUNGUS WITH 
OTHER MEMBERS OF THE SECTION ELEGANS 
The tii-st sets of j^iarallel cultures were intended to serve in 
the separation of any or all of the sj^ecies of Fusarium causing 
wilt from the soy bean fungus. F. dlseolor. var. sul phureum. 
F. oxi/sporunu F. raslnfeefum, F. li/eopersleL F. nlceutn, F. tra- 
ehel phll um . and Fusarluin sj). from soy bean were therefore 
grown on the following media, several sets of 10 cultures of each 
speci(*s being us(‘d on each medium: Potato ])lugs, steamed rice, 
cotton st(*ms, j)otato liard agar, and string-bean hard agar. The 
cultures were examined when 8, 15, 10, 30, and 50 days old. The 
iH‘Sults art‘ noti‘d in Table 1. Only those characters are recorded 
that are ii(‘c(‘ssarv tor the senaration of the snecies. 
