Hemorrhagic Septicemia Vaccines and Bacterins 
Sample No. 176 
A preparation sold as “Hemorrhagic Septicemia Bacterin 
for Cattle.” The test gave the results shown in Table IV. 
TABLE IV 
Rabbit 
No. 
Comrr 
bact 
No. 
2 c 
lercial 
;erin 
176 
:.c. 
Injection of 
one loopful 
of culture 
B. bipolaris 
No. 156 
2/28 
Dates 
of 
deaths 
Remarks 
2/17 
2/21 
1 
— 
— 
X 
3/1 
2 
3 
X 
X 
X 
X 
3/1 
3/1 
Subject in poo* condition, 
f Survived injection of strain 
4 
X 
X 
X 
3/10 
1 156, but when injected3/8 
\ with strain 59 it died two 
{ days later. 
5 
X 
X 
X 
3/1 
f Survived injection of strain 
6 
X 
X 
X 
3/10 
j 156, but when injected on 
I 3/8 with strain 59 it died 
7 
X 
X 
X 
3/2 
[ two days later. 
8 
X 
X 
X 
3/4 
9 
X 
X 
X 
3/3 
10 
X 
1 
X 
X 
3/4 
Sample No. 359. 
This preparation was sold to us as “Hemorrhagic Septicemia 
Combined Bacterin (Bovine).” The immunity of the rabbits 
treated with this substance was tested with three strains of B. 
bipolaris of bovine origin. It will be noted that the virulence of 
one of those strains was lost and that the rabbits with one ex- 
ception succumbed to a subsequent infection. For details of this 
experiment see Tables V, VI and VII. 
