10 Nebraska Experiment Station Research Bulletin IT 
Sample No. 485. 
A product sold as “Bacillus Suisepticus Bacterin (for swine 
plague) .” 
As the manufacturer of this material in common with others, 
indicates in his advertising matter that repeated bacterin injec- 
tions are desirable, we designed this series of experiments so 
that three groups of six rabbits each received respectively one. 
two and three bacterin injections while another group of ten 
rabbits were injected not less than twelve times. All injections 
were made every other day and were so timed that the entire 
series received its last immunizing dose on the same day. 
Ten days later each of the rabbits in this series 'received an 
inoculation with 1/10 c.c. of virus mixture prepared by taking 
the heart blood of a rabbit dead as a result of inoculation with 
B. bipolaris (from swine) mixed with twenty parts of salt solu- 
tion and added to equal parts of a 24-hour boullion culture of 
the same organism. Table IX shows the results of those trials. 
