4 Nebraska Experiment Station Research Bulletin 18 
From those various manufacturers we purchased a quantity 
of their products, treated a given number of fowls with them 
and then tested the immunity of the latter by means of inocula- 
tions with fowl cholera organisms secured from field outbreaks 
and cultivated in our laboratory. 
We hereby submit the details of those experiments in the 
following tables. 
Sample No. 148. 
This preparation was sold to us as “Hemorrhagic Septicemia 
Vaccine (Avian).” The details of the tests of this material are 
given in Table I. 
TABLE I 
Chicken 
No. 
' 
Fowl cholera 
vaccine No. 148 
2/3 
Quantity 1 c.c. 
One loopful 
culture 
B. bipolaris 
avisepticus 
2/11 
Strain 38 B, 
Dates of 
deaths 
Remarks 
1 

X 
2/12 
All chickens were very 
2 
— 
X 
2/14 
sick the day after the 
3 
X 
X 
2/14 
virus injection. 
4 
X 
X 
2/13 
5 
X* 
X 
Very sick. Recovered 
6 
X 
X 
2/23 
7 
X 
X 
2/17 
8 
X 
X 
2/13 
9 
X 
X 
Very sick. Recovered 
10 
X 
X 
2/15 
11 
X 
X 
Very sick. Recovered 
12 
X 
X 
2/15 
NOTE — The fact that an Injection was made is indicated by the X. A dash 
( — ) t s used to show that no injection took place. 
