148 Proceedings of Royal Society of Edinburgh. [sess. 
The remaining fish was living next day. (24 hours.) 
Oxygen =1 cc. per litre. T. 13° C. 
After 48 hours it was still alive. Oxygen = 1 cc. per litre. T. 
11° C. 
The fish in the flask seemed perfectly well after 48 hours. 
Oxygen = 4 cc. per litre. T. 13° C. 
At 10.20 on the third day two small fish from the flask were 
placed in A. One died at 10.30, and at 10.45 the last fish from 
the flask was placed in the bottle. It was lying on its side gasping 
at 11.25, and though placed in oxygenated water at 12 it died. 
(75 minutes.) The other was removed. 
The water in the bottle was shaken up with air at 12.30, when 
there was 4 cc. oxygen per litre. At 1 p.m. two fish of 3 grm. 
and 1*5 grm. were put in the water. At 11.45 p.m. both were 
living, but at 10 a.m., after 22 hours, they were both dead, and the 
water contained 0 cc. oxygen per litie. 
Experiment IV . — The Wolff’s bottle A. was filled to 2400 cc. 
and a flask B. was filled with 2800 cc. water. The water from the 
bottle used in the last experiment was filtered, and after the 
amount of oxygen had been found to be 2 cc. per litre it was 
divided into two portions of 700 cc., of which C 1 was left un- 
oxygenated, and C 2 was shaken with air, and a fish placed in each 
at 12.40. At 10.30 next morning, T. 13° C. — 
A. contained 3 cc. oxygen per litre. 
T> £ 
- 1 -** 55 U ,, ,, ,, 
C 2 
vy * 15 5 5 5 5 5 5 
At 12.40— 
Three fish were placed in A. 
Two fish ,, ,, B. 
A. On the morning of next day the two smaller fish were 
dead. Oxygen = 1 cc. per. litre. T. 14° C. 
The remaining fish was quite well on the following day at 
12.55, when the water contained 1 cc. oxygen per litre. After 
72 hours it was put in oxygenated water and was very lively. 
