19j1— 2.] Experimental Observations on Leucolysis. 291 
protoplasm swells, loses its outline, shows more marked vacuolation, 
*md instead of granulation a diffuse pale pink colour (with eosin 
and methylene-blue). Further appearances vary according to the 
number of organisms taken up by the cells, Many are so 
engorged that they show only an indistinct nucleus and a faint 
•cell border, and some are represented only by fragments of a 
nucleus surrounded by a hunch of cocci. Other cells containing 
only a few organisms or none at all show all gradations, from 
wacuolation, raggedness, and swelling of the protoplasm with 
wacuolation and indistinctness of the nucleus, to disintegration into 
a granular debris. 
Lymphocytes . — -The large lymphocytes also exhibited phago- 
cytosis, but there was no definite evidence of cliemiotaxis. The 
number of organisms ingested was much fewer than that taken up 
by the polymorphonuclear cells, the average being four or five, and 
the maximum never having been noticed to exceed twenty. It was 
quite exceptional to find a large lymphocyte which did not show 
degenerative change. Fraying or budding of the protoplasmic 
border is usually the first change to occur. Along with this, err in 
some cases preceding it, there is vacuolation of the protoplasm. 
The cell then swells up, nucleus and protoplasm appear to fuse, 
vacuolation becomes extreme, and total disintegration follows. 
The small Lymphocytes . — Yery rarely a small lymphocyte which 
had ingested one or two organisms was seen, but this was quite 
•exceptional. The small lymphocytes showed the least degree of 
necrobiotic change ; and in some films where every other white cell 
had been disintegrated, normal lymphocytes could be found. The 
necrobiotic changes they show are fraying and budding of their 
border, and occasionally vacuolation of their protoplasm. The 
nucleus is very resistant, but eventually becomes vacuolated and 
loses its shape before disintegrating. It is interesting to note that 
Gulland (3) finds in pleural effusions that “the lymphocytes do 
not appear to degenerate wffth anything like the same frequency 
as the polymorphonuclear cells.’’ 
The Eosinophiles . — The normal scarcity of these cells, the readi- 
ness with which they are broken up, and their resemblance to the 
neutrophiles in the later stages of disintegration make the study of 
the eosinophiles particularly difficult. The examination of sputum 
