676 Proceedings of Royal Society of Edinburgh. [sess. 
with eosine, and are therefore albuminous in nature, but not so 
strongly so as the zymogen granules. 
The chromatin stains of an ultramarine blue, due to its acid 
nature, and is arranged in part on the inner surface of the nuclear 
envelope, and in part in more or less isolated rounded or irregular 
karyosomes. 
(2) Twenty hours after food . — (Worm partly digested.) (Fig. 2, 
coloured plate, photo No. 2.) — The cells are small, in some cases 
almost flattened, and the gland lumen is correspondingly large and 
stellate. 
The zymogen granules have nearly disappeared from the proto- 
plasm, those remaining being of large size, 2T6 /x in diameter, 
with an average of 1*6 /x. They are situated mostly near the free 
ends of the cells, leaving the base and sides clear. This gives rise 
to the appearance of two zones in the cells, the one basal, contain- 
ing the nucleus, and the other apical, containing the granules. 
Only a few cells, however, show this division into zones ; most of 
them have a few granules scattered throughout the protoplasm. 
No zymogen granules are visible in the lumen, from which I con- 
clude that the granules dissolve in the protoplasm before the 
secretion leaves the cell, which it does in a liquid and unstainable 
form, the protoplasm appears condensed and stains pale blue, the 
condensation being due rather to the disappearance of the granules 
and discharge of the fluid in the vacuoles than the reconstruction 
of the protoplasm. In this I agree rather with Nicolas (20) than 
with Langley (13). 
The nuclei are larger at this time than in the newt that was 
killed ten days after food, the average of a number of measure- 
ments being 1 1 '85 x 1 3 '3 6 /x. The nuclear membrane appears 
thickened, owing probably to the chromatin spreading itself out 
upon the inner surface. There is usually a great dearth of chromatin 
deeper in, and that which is present is pale and has a washed-out 
appearance. 
The lanthanin granules have in the majority of cases undergone 
no change, but in a few nuclei they stain of a deeper tint. The 
nucleoli vary a good deal in size, but are not numerous. They 
stain of the same venous tint, and the larger ones may often be 
seen lying quite close to the nuclear membrane, and some of them 
