1888 - 89 .] Messrs Macleod and Milles on Asiatic Cholera. 31 
Three animals, after injection into the stomach of soda solution 
alone, seemed in no way affected. 
From one of the animals that died after a dose of cholera material, 
the small gut contents were collected in a sterilised vessel, and 
injected in doses of 2 c.cm. into the stomachs of two other animals. 
These two animals died, and the contents of their small intestine 
were used in the same way, and so on through ten generations. Of 
twenty-one animals thus treated two recovered, nineteen died. The 
dose varied from 05 to 2*5 c.cm. 
As control experiments to these, two animals received 5 c.cm. of 
the soda solution, 5 c.cm. of sterilised broth, and thereafter the usual 
dose of opium tincture. After six hours these animals were killed, 
and the contents of their small intestine collected and injected with 
the same precautions in doses of 2 c.cm. Four animals thus treated 
all recovered. 
These results with bowel contents dispose of Klein’s objection 
that the action of “ a chemical poison present in certain cultivations 
of the cholera comma bacillus,” is necessary to kill guinea pigs, since 
no cultivations were used. 
The caecum of the cholera-infected animals w T as usually distended 
with watery, and if the animal died early, greenish contents, and 
contained comma bacilli in great numbers, among a multitude of 
other forms. From an animal where this was the case, 2 c.cm. of 
the contents of the ileum were injected into the stomach of one 
animal, and a similar quantity of the contents of the caecum into 
that of each of the three others. The former died ; the three latter 
recovered without a sign of sickness. Unfortunately no plate culti- 
vations were made of those caecal contents to ascertain whether the 
comma bacilli seen there were capable of growth like those of the 
ileum. Koch’s contention that these organisms are killed in the 
caecum of these animals is so far borne out by these three experi- 
ments. 
Of the fifteen animals that recovered after injection of the cholera 
material, seven were manifestly sick, two were slightly so, and six 
were not apparently affected. 
At the present time even Koch’s most energetic opponent (Klein) 
will allow that the proof of Asiatic cholera in man is the presence of 
the comma bacillus either in the stools of a person taken suddenly 
