1888-89.] Haycraft and Duggan on Coagulation by Heat. 377 
(c) Vitellin, dissolved in 10 per cent, solution of common salt, 
"became opalescent at 80° C., and coagulated at 86° C. 
( d ) Vitellin, dissolved in 5 per cent, solution of common salt, 
became opalescent at 79° C., and coagulated at 85° C. 
(e) Vitellin, dissolved in 2 ‘5 per cent, solution of common salt, 
became opalescent at 78° C., and coagulated at 83° C. 
This experiment was repeated, and showed that common salt 
raises the coagulation point of vitellin, but that it is lowered just 
before the point of saturation, and that it continues to be lowered 
until saturation occurs. 
Action on the Coagulation Point of Vitellin of both Common Salt 
and Magnesium Sulphate dissolved together in the Solution , 
If, to vitellin in a saturated solution of common salt, some 
magnesium sulphate be added, the latter dissolves with difficulty, 
precipitating the vitellin in flocculi ; on heating, other flocculi 
appear. 
If, to vitellin in a saturated solution of magnesium sulphate, 
common salt be added, the coagulation point is lowered. Thus, 
on adding 15 per cent, of common salt, coagulation occurs at 88° C., 
and with a little over 20 per cent, it is lowered to 70° C. 
Serum Globulin. — Serum globulin is precipitated by magnesium 
sulphate in excess, as Hammarsten has shown. The same observer 
obtained a precipitation by saturating with common salt. 
The Action of Magnesium Sulphate on the Coagulation Point of 
Serum Globulin. 
Serum globulin was precipitated from the serum of ox’s blood by 
passing a stream of C0 2 through it. The precipitate after careful 
washing was dissolved in magnesium sulphate solution. 
(a) Serum globulin is precipitated in the cold by saturating the 
solution with magnesium sulphate. 
(b) Serum globulin, dissolved in a solution containing 50 per 
cent, magnesium sulphate, became opalescent at 7 4° *5 C., and 
coagulated at 79° C. 
(c) Serum globulin, dissolved in a solution containing 25 per 
cent, magnesium sulphate, became opalescent at 78°*5 C., and 
coagulated at 80° *75 C. 
