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After 3 days 3 or 4 small spheres appeared, containing 
living bacteria. 
After 4 days spheres enlarged and a deposit forming at 
the bottom of them very full of bacteria. 
After 6 days the surface of the jelly was beginning to 
give way. 
After 8 days the surface layer was liquid to the depth of 
a millimetre, but the rest of the gelatine was still firm, with 
no smell ; liquid layer alkaline. 
Specimen 2.- — 25 cc. gelatine + 25 cc. Manchester water 
4- 5 mgms. sodium phosphate. 
After 1 day the tube contained a few minute specks. 
After 2 days a number of minute spheres, not to be 
counted, appeared, and a turbid band was formed near the 
surface of the jelly, which was very rich in living bacteria. 
After 3 days spheres became larger and the whole of the 
gelatine was softened ; a number of air bubbles appeared 
also. 
After 4 days air spheres had risen to surface, bacteria 
spheres disappeared, leaving the liquid jelly turbid and 
smelling offensively ; liquid alkaline. 
Specimen 3. — 25 cc. gelatine + 25 cc. Manchester water 
+ 5 mgms. sodium phosphate after filtration through spongy 
iron. 
The result here resembled that of the distilled water. 
25 cc. gelatine + 25 cc. Mersey water at Northenden + 
5 mgms. sodium phosphate. 
After 1 day a turbid band appeared near the surface and 
a number of minute spots appeared. 
After 2 days these spots had spheres about them. 
After 3 days the surface layer was quite liquid and turbid 
and a number of discs of gas appeared. Bacteria in spots 
and surface liquid. 
After 4 days in a somewhat similar condition, but the 
surface liquid possessed a very disagreable smell. 
