69 
colonies, which gradually spread and form a thin, white granular film. 
margins wavy or sometimes slightly corrugated. The growth has a 
slight tendency to liquefy at a certain period of its development. The 
second produces a pink growth, such as has been noted and described 
in considering experiment 5. The third is a profuse beautiful yellow 
growth, beginning at first by dense, thick round colonies, rather whitish 
at first, but soon turning yellow. When fused and the growth pretty 
nearly completed it is nearly always quite thick and deep yellow, with 
margins entire or wavy. The first and third of these are the ones 
which seem to be constantly associated with the disease. The one 
producing the thin, white film is the one which is parasitic, or at least 
partially so, in its relations to Plusia brassicce. 
Pure cultures of this germ were also received June 3 from Dr. J. 0. 
Neal, Lake City, Fla. Upon unsealing the tubes a small amount of 
gas escaped with a fizz, accompanied by a smell reminding one of 
rotten eggs. Fresh agar cultures were made, and from these, liquid 
cultures were prepared for use in experiments C and 7. The culture 
liquid used in these experiments was eighteen days old. 
Experiment 6. 
June 27. — A cabbage leaf was drenched with the charged liquid, and four Pieris 
larv* placed on to feed. The day following; all were feeding briskly. Two days 
later the leaf had all been eaten and fresh food was placed in. No indications of 
disease three days afterward. Later three larvae pupated, one of which was acciden- 
tally injured and died. The fourth larva died, but did not rot or turn dark. It dried 
up gradually, which indicated that the pot had been too strongly disinfected and 
that the larva had been poisoned from crawling about its walls. No cultures were 
subsequently obtained from it. The two living pupa} hatched in due time. 
Experiment 7. 
June 27. — Two bolls were prepared as described in experiment 4, but using the 
same culture liquid designated for experiment 6, namely, the Plusia disease genu. 
Two Boll Worms were placed on, and each was subsequently observed sipping of 
the liquid. One of the larvae was full grown, and had shortened some, preparatory 
to pupation; the other was also about full grown, but fed until mature. Both sub- 
sequently entered the earth and completed their transformation without any diffi- 
culty. 
Checks during the progress of the experiments recorded, and many 
others were carefully continued. Since no results were obtained from 
the experiments requiring it, the consideration of the checking will, in 
this report, be superfluous, save to remark that disease did not appear 
in them in any instance, except experiments 2 and o, which have already 
been included. 
DISCUSSION OF THE RESULTS. 
Prom the beginning complicating conditions were discovered. The 
most important one was that the species in question (Heliothis armiger) 
was subject to a disease which was probably as prevalent as the nature 
